CAJ | 학술논문

目的：探讨真武汤对糖尿病大鼠肾脏的保护作用及机制。方法：腹腔注射链脲佐菌素（ STZ）建立糖尿病肾病大鼠模型，将成模大鼠随机分为糖尿病肾病（ DN）模型组和真武汤治疗组（真武组），另设正常组。采用生化、HE染色观察真武汤对糖尿病肾病大鼠肾功能、肾组织形态学变化及脂质过氧化相关参数的作用；采用蛋白免疫印迹方法探讨真武汤对肾组织α-平滑肌肌动蛋白（α-SMA）和NF-κB表达的影响。结果：模型组大鼠肾系数、24 h尿蛋白定量、血尿素氮、肌酐、血糖和丙二醛（MDA）均显著升高（P＜0．05），体重、超氧化物歧化酶（SOD）及诱导型一氧化氮合酶（iNOS）显著降低（P＜0．05）；真武组大鼠的肾系数、24 h尿蛋白、尿素氮、肌酐、血糖和MDA明显低于模型组（P＜0．05），iNOS显著高于模型组（P＜0．05）；模型组大鼠肾小球肥大、毛细血管基底膜增厚，系膜基质增生，肾小管上皮细胞空泡样变，可见蛋白管型；真武组病变轻于模型组。模型大鼠肾组织α-SMA及NF-κB蛋白的水平明显高于正常组（P＜0．05），真武组大鼠肾组织α-SMA及NF-κB蛋白水平明显低于模型组（P＜0．05）。结论：真武汤能减轻糖尿病肾病肾脏局部氧化应激反应，改善糖尿病肾病大鼠肾功能，减轻病理损伤，其发挥肾脏保护作用可能与抑制α-SMA及NF-κB蛋白的表达有关。
목적：탐토진무탕대당뇨병대서신장적보호작용급궤제。방법：복강주사련뇨좌균소（ STZ）건립당뇨병신병대서모형，장성모대서수궤분위당뇨병신병（ DN）모형조화진무탕치료조（진무조），령설정상조。채용생화、HE염색관찰진무탕대당뇨병신병대서신공능、신조직형태학변화급지질과양화상관삼수적작용；채용단백면역인적방법탐토진무탕대신조직α-평활기기동단백（α-SMA）화NF-κB표체적영향。결과：모형조대서신계수、24 h뇨단백정량、혈뇨소담、기항、혈당화병이철（MDA）균현저승고（P＜0．05），체중、초양화물기화매（SOD）급유도형일양화담합매（iNOS）현저강저（P＜0．05）；진무조대서적신계수、24 h뇨단백、뇨소담、기항、혈당화MDA명현저우모형조（P＜0．05），iNOS현저고우모형조（P＜0．05）；모형조대서신소구비대、모세혈관기저막증후，계막기질증생，신소관상피세포공포양변，가견단백관형；진무조병변경우모형조。모형대서신조직α-SMA급NF-κB단백적수평명현고우정상조（P＜0．05），진무조대서신조직α-SMA급NF-κB단백수평명현저우모형조（P＜0．05）。결론：진무탕능감경당뇨병신병신장국부양화응격반응，개선당뇨병신병대서신공능，감경병리손상，기발휘신장보호작용가능여억제α-SMA급NF-κB단백적표체유관。
AIM:To investigate the effects of Zhenwu decoction ( ZWD) on the expression of α-smooth muscle actin (α-SMA) and NF-κB in diabetic nephropathy ( DN) rats.METHODS:Diabetic rat model was induced by intrape-ritoneal injection of streptozotocin ( STZ) , and the animals were randomly divided into STZ group ( n=22) and STZ+ZWD group (n=23).The normal rats served as control (n=16).All rats were sacrificed on 8 weeks after modeling.Biochemical assay and pathological observation ( HE staining and transmission electron microscopy ) were used to evaluate the effects of Zhenwu decoction on the renal function and pathological morphology .The body weight , renal index , blood glucose , total urinary protein in 24 h, and superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) , inducible nitric oxide synthase ( iNOS) were determined as well .Western blotting was used to observe the effects of Zhenwu decoction on the expression ofα-SMA and NF-κB in diabetic nephropathy ( DN) rats.RESULTS:Compared with normal group , the renal index , blood glucose concentration, total urinary protein in 24 h, blood urea nitrogen (BUN), serum creatinine (SCr) and MDA were significantly higher and body weight was lower in DN rats (P<0.05).Pathological examination of the kidneys in DN group showed glomerular hypertrophy , glomerular basement membrane thickening , tubular epithelial cell degeneration , me-sangial matrix proliferation , protein cast formation in some renal tubules .The protein expression levels of α-SMA and NF-κB were markedly increased (P<0.05).After ZWD treatment, the level of renal index, total urinary protein in 24 h, BUN, SCr and the expression of α-SMA and NF-κB at the protein level were significantly decreased (P<0.05).The renal histological injury in ZWD group was significantly ameliorated .CONCLUSION: Zhenwu decoction might protect kidney against STZ-induced injury via decreasing the expression of α-SMA and NF-κB.