中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
9期
1666-1671
,共6页
施庆颜%靳华%曾靖华%邝少华%黄世光
施慶顏%靳華%曾靖華%鄺少華%黃世光
시경안%근화%증정화%광소화%황세광
慢性根尖周病%肥大细胞%白细胞介素类
慢性根尖週病%肥大細胞%白細胞介素類
만성근첨주병%비대세포%백세포개소류
Chronic periapical disease%Mast cells%Interleukins
目的：观察不同类型慢性根尖周病组织中肥大细胞（ mast cells， MCs）的分布情况，并分析白细胞介素1β（interleukin-1β，IL-1β）和白细胞介素17（interleukin-17，IL-17）在MCs上的表达情况，探讨MCs IL-1β和IL-17在根尖周病发病机制中的作用。方法：本实验共收集102例样本，分为3组：（1）根尖肉芽肿组32例；（2）根尖囊肿组35例；（3）正常对照组35例。组织标本经4％中性甲醛缓冲液固定48 h以上；石蜡包埋，组织连续切片，HE染色，在光学显微镜下观察其组织学改变；采用免疫荧光双染色法，在荧光显微镜下观察各组标本组织表达IL-1β和IL-17的MCs数目。结果：两组慢性根尖周病的炎症反应程度明显高于正常对照组（ P＜0．01）；根尖囊肿组与根尖肉芽肿组之间炎症反应程度无显著差异（P＞0．05）。与正常对照组相比，两组慢性根尖周病组织中IL-1β和IL-17阳性MCs数目均显著增多（P＜0．01）；根尖囊肿组与根尖肉芽肿组IL-1β和IL-17阳性MCs密度无显著性差异（P＞0．05）。经Pearson相关分析，3组标本组织中IL-1β和IL-17阳性MCs密度与组织炎症反应程度存在直线的正相关关系（P＜0．01）。结论：慢性根尖周病组织中MCs数目明显增多，同时IL-1β和IL-17阳性的MCs密度显著增高。 IL-1β和IL-17阳性MCs密度与根尖周病的炎症程度趋势相一致。这提示IL-1β和IL-17阳性MCs可能在慢性根尖周病的致病机制中发挥重要作用。
目的：觀察不同類型慢性根尖週病組織中肥大細胞（ mast cells， MCs）的分佈情況，併分析白細胞介素1β（interleukin-1β，IL-1β）和白細胞介素17（interleukin-17，IL-17）在MCs上的錶達情況，探討MCs IL-1β和IL-17在根尖週病髮病機製中的作用。方法：本實驗共收集102例樣本，分為3組：（1）根尖肉芽腫組32例；（2）根尖囊腫組35例；（3）正常對照組35例。組織標本經4％中性甲醛緩遲液固定48 h以上；石蠟包埋，組織連續切片，HE染色，在光學顯微鏡下觀察其組織學改變；採用免疫熒光雙染色法，在熒光顯微鏡下觀察各組標本組織錶達IL-1β和IL-17的MCs數目。結果：兩組慢性根尖週病的炎癥反應程度明顯高于正常對照組（ P＜0．01）；根尖囊腫組與根尖肉芽腫組之間炎癥反應程度無顯著差異（P＞0．05）。與正常對照組相比，兩組慢性根尖週病組織中IL-1β和IL-17暘性MCs數目均顯著增多（P＜0．01）；根尖囊腫組與根尖肉芽腫組IL-1β和IL-17暘性MCs密度無顯著性差異（P＞0．05）。經Pearson相關分析，3組標本組織中IL-1β和IL-17暘性MCs密度與組織炎癥反應程度存在直線的正相關關繫（P＜0．01）。結論：慢性根尖週病組織中MCs數目明顯增多，同時IL-1β和IL-17暘性的MCs密度顯著增高。 IL-1β和IL-17暘性MCs密度與根尖週病的炎癥程度趨勢相一緻。這提示IL-1β和IL-17暘性MCs可能在慢性根尖週病的緻病機製中髮揮重要作用。
목적：관찰불동류형만성근첨주병조직중비대세포（ mast cells， MCs）적분포정황，병분석백세포개소1β（interleukin-1β，IL-1β）화백세포개소17（interleukin-17，IL-17）재MCs상적표체정황，탐토MCs IL-1β화IL-17재근첨주병발병궤제중적작용。방법：본실험공수집102례양본，분위3조：（1）근첨육아종조32례；（2）근첨낭종조35례；（3）정상대조조35례。조직표본경4％중성갑철완충액고정48 h이상；석사포매，조직련속절편，HE염색，재광학현미경하관찰기조직학개변；채용면역형광쌍염색법，재형광현미경하관찰각조표본조직표체IL-1β화IL-17적MCs수목。결과：량조만성근첨주병적염증반응정도명현고우정상대조조（ P＜0．01）；근첨낭종조여근첨육아종조지간염증반응정도무현저차이（P＞0．05）。여정상대조조상비，량조만성근첨주병조직중IL-1β화IL-17양성MCs수목균현저증다（P＜0．01）；근첨낭종조여근첨육아종조IL-1β화IL-17양성MCs밀도무현저성차이（P＞0．05）。경Pearson상관분석，3조표본조직중IL-1β화IL-17양성MCs밀도여조직염증반응정도존재직선적정상관관계（P＜0．01）。결론：만성근첨주병조직중MCs수목명현증다，동시IL-1β화IL-17양성적MCs밀도현저증고。 IL-1β화IL-17양성MCs밀도여근첨주병적염증정도추세상일치。저제시IL-1β화IL-17양성MCs가능재만성근첨주병적치병궤제중발휘중요작용。
AIM:To identify and quantify the expression of IL-1βand IL-17 in mast cells ( MCs) in different types of human pericapical diseases using double immunofluorescence staining .METHODS: The specimens ( n=102 ) , including healthy control ( n=35 ) , periapical cyst ( n=35 ) and periapical granuloma ( n=32 ) , were involved in the present study .The tissue samples were fixed in 10% buffered formalin for at least 48 h and then embedded in paraffin . Serial 5-μm-thick sections were deposited onto SuperFrost/Plus microscope glasses .Routine staining of the sections using hematoxylin&eosin ( HE) was performed for morphological evaluation .The number of IL-1βand IL-17 positive MCs was identified by double immunofluorescence staining .RESULTS:Compared with the healthy controls , the inflammation score of periapical lesions was significantly increased in the periapical patients (P<0.01).The density of IL-1βand IL-17 posi-tive MCs in the periapical lesions were obviously higher than that in the healthy controls (P<0.01).However, no signifi-cant difference between periapical cyst and periapical granuloma was observed .The Pearson correlation analysis showed that there was a positive correlation between the density of IL-1βand IL-17 double positive MCs and inflammation score in dif-ferent groups of specimens (P<0.01).CONCLUSION:There is significantly increased number of MCs , along with in-creased density of IL-1βand IL-17 positive MCs in human periapical lesions .The increased density of IL-1βand IL-17 positive MCs has the similar tendency as the severity of tissue inflammation in human periapical lesions , suggesting that IL-1βand IL-17 positive MCs may play an important role in the pathogenesis of human periapical diseases .
