CAJ | 학술논문

目的：观察梓醇促进大鼠骨髓间充质干细胞（ bone marrow mesenchymal stem cells ， BMSCs ）增殖过程中Wnt信号通路的变化。方法：（1）采用机械分离及差速贴壁法分离培养SD大鼠BMSCs，将细胞分为空白对照组与梓醇（1．0 mg/L）处理组，流式细胞术检测各组细胞细胞周期分布情况，计算其增殖指数。（2）实时荧光定量PCR法检测各组细胞中Wnt3a、Wnt5a、Wnt11及β-catenin mRNA的表达水平；Western blotting技术检测各组细胞β-catenin蛋白的表达变化。结果：（1）空白对照组与梓醇处理组BMSCs增殖指数分别为8.90％±0.46％和17.93％±1.68％（P＜0.01）。（2）与空白对照组相比，梓醇处理组Wnt5a、Wnt11、β-catenin mRNA及β-catenin蛋白表达均明显提高，但Wnt3a mRNA表达无显著变化。结论：梓醇在促进BMSCs增殖过程中可同时激活经典与非经典Wnt信号通路。
목적：관찰재순촉진대서골수간충질간세포（ bone marrow mesenchymal stem cells ， BMSCs ）증식과정중Wnt신호통로적변화。방법：（1）채용궤계분리급차속첩벽법분리배양SD대서BMSCs，장세포분위공백대조조여재순（1．0 mg/L）처리조，류식세포술검측각조세포세포주기분포정황，계산기증식지수。（2）실시형광정량PCR법검측각조세포중Wnt3a、Wnt5a、Wnt11급β-catenin mRNA적표체수평；Western blotting기술검측각조세포β-catenin단백적표체변화。결과：（1）공백대조조여재순처리조BMSCs증식지수분별위8.90％±0.46％화17.93％±1.68％（P＜0.01）。（2）여공백대조조상비，재순처리조Wnt5a、Wnt11、β-catenin mRNA급β-catenin단백표체균명현제고，단Wnt3a mRNA표체무현저변화。결론：재순재촉진BMSCs증식과정중가동시격활경전여비경전Wnt신호통로。
AIM:To investigate the changes of Wnt signaling pathway in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells (BMSCs).METHODS:The BMSCs were isolated from SD rats , purified by differ-ential time adherent method and divided into control group and catalpol (1.0 mg/L) group.Flow cytometry was used to de-tect the proliferation index of BMSCs .The mRNA levels of Wnt3a, Wnt5a, Wnt11 and β-catenin was evaluated by real-time PCR.In addition, the protein expression level of β-catenin was determined by Western blotting .RESULTS:Prolife-ration index was increased from 8.90%±0.46% to 17.93%±1.68% after treatment with catalpol (P<0.01).Com-pared with control group , the mRNA expression of Wnt5a, Wnt11 andβ-catenin was all increased with catalpol treatment . No difference of Wnt3a mRNA expression between control group and catalpol group was observed .Meanwhile, the protein expression of β-catenin was increased in catalpol group compared with control group .CONCLUSION:Catalpol promotes BMSCs going into the cell cycle .Classical and non-classical Wnt signaling pathways are activated in this process .