CAJ | 학술논문

背景：胰腺癌发病隐匿，进展迅速，预后极差。光动力疗法（ PDT）是20世纪80年代发展起来的一种新型抗肿瘤治疗手段。目前关于PDT在体内靶向治疗胰腺癌的研究甚少。目的：探讨以量子点-RGD（ QDs-RGD）探针为光敏剂的PDT联合吉西他滨对胰腺癌移植瘤裸鼠的治疗效应。方法：合成QDs-RGD探针，制备胰腺癌移植瘤裸鼠模型。采用小动物活体成像术观察QDs-RGD、QDs探针注射入模型裸鼠体内1、5、10、24 h后的显影情况。取40只造模成功的裸鼠，随机分为5组：对照组（不给予任何治疗）；单纯光照组（激光630 nm，120 J/cm2，持续照射20 min）；PDT组（QDs-RGD 0.5 nmol+激光照射）；吉西他滨组（吉西他滨50 mg/kg）；联合治疗组（QDs-RGD 0.5 nmol+激光照射+吉西他滨50 mg/kg）。第18 d处死全部裸鼠，取出瘤体，称重并测量体积，计算抑瘤率。结果：QDs-RGD注射1 h后，肿瘤显影逐渐清晰，注射5 h后显影达高峰，随后逐渐减弱。QDs于瘤体附近的聚集浓度明显低于QDs-RGD，注射10 h后肿瘤部位已无显影。PDT组、吉西他滨组和联合治疗组的瘤重、瘤体积均显著低于对照组和单纯光照组（P<0.01），其中联合治疗组又显著低于PDT组和吉西他滨组（P<0.05）；对照组与单纯光照组间、PDT组与吉西他滨组间瘤重、瘤体积差异均无统计学意义（ P>0.05）。联合治疗组、吉西他滨组、PDT组的抑瘤率分别为70.5%、43.5%、37.1%。结论：以QDs-RGD探针为光敏剂的PDT联合吉西他滨能明显抑制裸鼠体内胰腺癌移植瘤的生长，为临床治疗胰腺癌提供了一条新思路。
배경：이선암발병은닉，진전신속，예후겁차。광동력요법（ PDT）시20세기80년대발전기래적일충신형항종류치료수단。목전관우PDT재체내파향치료이선암적연구심소。목적：탐토이양자점-RGD（ QDs-RGD）탐침위광민제적PDT연합길서타빈대이선암이식류라서적치료효응。방법：합성QDs-RGD탐침，제비이선암이식류라서모형。채용소동물활체성상술관찰QDs-RGD、QDs탐침주사입모형라서체내1、5、10、24 h후적현영정황。취40지조모성공적라서，수궤분위5조：대조조（불급여임하치료）；단순광조조（격광630 nm，120 J/cm2，지속조사20 min）；PDT조（QDs-RGD 0.5 nmol+격광조사）；길서타빈조（길서타빈50 mg/kg）；연합치료조（QDs-RGD 0.5 nmol+격광조사+길서타빈50 mg/kg）。제18 d처사전부라서，취출류체，칭중병측량체적，계산억류솔。결과：QDs-RGD주사1 h후，종류현영축점청석，주사5 h후현영체고봉，수후축점감약。QDs우류체부근적취집농도명현저우QDs-RGD，주사10 h후종류부위이무현영。PDT조、길서타빈조화연합치료조적류중、류체적균현저저우대조조화단순광조조（P<0.01），기중연합치료조우현저저우PDT조화길서타빈조（P<0.05）；대조조여단순광조조간、PDT조여길서타빈조간류중、류체적차이균무통계학의의（ P>0.05）。연합치료조、길서타빈조、PDT조적억류솔분별위70.5%、43.5%、37.1%。결론：이QDs-RGD탐침위광민제적PDT연합길서타빈능명현억제라서체내이선암이식류적생장，위림상치료이선암제공료일조신사로。
Background:Pancreatic cancer is obscure in onset and progresses rapidly with very poor prognosis. Photodynamic therapy( PDT)has been developed as a novel anti-tumor treatment modality since 1980s. At present,there are only limited researches on pancreatic cancer treated with PDT in vivo. Aims:To investigate the efficacy of quantum dots-RGD ( QDs-RGD)based PDT combined with gemcitabine for treatment of pancreatic cancer xenograft in nude mice. Methods:QDs-RGD probe was synthesized and nude mice bearing pancreatic cancer xenograft was established. Nude mice were imaged at 1,5,10 and 24 hours after injection of QDs-RGD and QDs by in vivo imaging system. Forty model nude mice were randomly divided into five groups:control group( without any treatment),simple illumination group( laser 630 nm, 120 J/cm2,20 min),PDT group(QDs-RGD 0. 5 nmol+laser irradiation),gemcitabine group(gemcitabine 50 mg/kg)and combination group(QDs-RGD 0. 5 nmol+laser irradiation+gemcitabine 50 mg/kg). All the nude mice were sacrificed 18 days later. Tumor weight and volume were measured and tumor inhibition rate was calculated. Results:Fluorescence of tumor was shown 1 hour after injection and became clearest at the 5th hour,then showing a decrescendo trend. Density of QDs surrounding tumor was significantly less than that of QDs-RGD and faded away at the 10th hour. Tumor weight and volume in PDT group,gemcitabine group and combination group were all significantly lower than those in control group and simple illumination group(P<0. 01),and those in combination group were significantly lower than those in PDT group and gemcitabine group(P <0. 05). No significant differences in tumor weight and volume were found between control group and simple illumination group(P >0. 05),as well as between PDT group and gemcitabine group(P >0. 05). Tumor inhibition rate in combination group,gemcitabine group and PDT group was 70. 5%,43. 5% and 37. 1%, respectively. Conclusions:QDs-RGD based PDT combined with gemcitabine can inhibit the growth of pancreatic cancer xenograft in nude mice,which introduces a new idea to the treatment of pancreatic cancer.