山东医学高等专科学校学报
山東醫學高等專科學校學報
산동의학고등전과학교학보
JOURNAL OF SHANDONG MEDICAL COLLEGE
2014年
5期
364-365,366
,共3页
阿司匹林%水杨酸%阿司匹林酯酶%热稳定性
阿司匹林%水楊痠%阿司匹林酯酶%熱穩定性
아사필림%수양산%아사필림지매%열은정성
Aspirin%Salicylic acid%Aspirin esterase%Thermal stability
目的:本实验探究大鼠肝微粒体匀浆中阿司匹林酯酶的热稳定性。方法将大鼠肝微粒体匀浆分别在30、40、50℃的条件下处理后作为酶来源进行体外培养,以RP-HPLC法测定培养体系中阿司匹林(ASA)和水杨酸(SA)的浓度,以CSA/(CASA+ CSA)表示阿司匹林酯酶的活性。结果大鼠肝微粒体匀浆中阿司匹林酯酶在50℃下保存10min活性基本消失,仅剩15%;在40℃的条件下,保存60min后酶活性降低至20%,在30℃保存60min大鼠肝微粒体匀浆中阿司匹林酯酶活性约为60%。结论大鼠肝微粒体匀浆中阿司匹林酯酶对热不稳定,需低温保存。
目的:本實驗探究大鼠肝微粒體勻漿中阿司匹林酯酶的熱穩定性。方法將大鼠肝微粒體勻漿分彆在30、40、50℃的條件下處理後作為酶來源進行體外培養,以RP-HPLC法測定培養體繫中阿司匹林(ASA)和水楊痠(SA)的濃度,以CSA/(CASA+ CSA)錶示阿司匹林酯酶的活性。結果大鼠肝微粒體勻漿中阿司匹林酯酶在50℃下保存10min活性基本消失,僅剩15%;在40℃的條件下,保存60min後酶活性降低至20%,在30℃保存60min大鼠肝微粒體勻漿中阿司匹林酯酶活性約為60%。結論大鼠肝微粒體勻漿中阿司匹林酯酶對熱不穩定,需低溫保存。
목적:본실험탐구대서간미립체균장중아사필림지매적열은정성。방법장대서간미립체균장분별재30、40、50℃적조건하처리후작위매래원진행체외배양,이RP-HPLC법측정배양체계중아사필림(ASA)화수양산(SA)적농도,이CSA/(CASA+ CSA)표시아사필림지매적활성。결과대서간미립체균장중아사필림지매재50℃하보존10min활성기본소실,부잉15%;재40℃적조건하,보존60min후매활성강저지20%,재30℃보존60min대서간미립체균장중아사필림지매활성약위60%。결론대서간미립체균장중아사필림지매대열불은정,수저온보존。
Objective To study the thermal stability of aspirin esterase in rat liver microsomes .Methods Culture of homogenate of rat liver microsomes was cultured as the source of enzyme in vitro after being at 30℃ ,40℃and 50℃ .The concentrations of aspirin and salicylic acid were determined by RP- HPLC and CSA/(CASA+CSA) was used to reflect the activity of aspirin esterase .Results The activity of aspirin es-terase in rat liver microsomes declined to 15% at 50 ℃ and remained for 10 minutes ,declined to 20% at 40℃ and remained for 60 minutes ,and declined to 60% at 30℃and remained for 60 minutes .Conclusion Aspirin esterase in rat liver microsomes shows thermal instability and needs to be preserved under low tem-perature .