CAJ | 학술논문

以人肺癌A549细胞为研究对象，探讨仙人掌多糖组分OP1对人肺癌A549细胞生长抑制，以及对其形态、结构的影响．采用台盼蓝拒染法测定人肺癌A549细胞生长抑制曲线，通过倒置显微镜观察不同质量浓度的仙人掌多糖组分OP1作用于人肺癌A549细胞引发的形态变化．结果表明，仙人掌多糖组分OP1能抑制人肺癌A549细胞增殖，诱导人肺癌A549细胞凋亡，在一定范围内，呈时间、剂量依赖性，作用48 h的IC50为（597.55±28.97）μg/mL．经仙人掌多糖组分OP1诱导后，人肺癌A549细胞形态结构出现典型的凋亡特征．
이인폐암A549세포위연구대상，탐토선인장다당조분OP1대인폐암A549세포생장억제，이급대기형태、결구적영향．채용태반람거염법측정인폐암A549세포생장억제곡선，통과도치현미경관찰불동질량농도적선인장다당조분OP1작용우인폐암A549세포인발적형태변화．결과표명，선인장다당조분OP1능억제인폐암A549세포증식，유도인폐암A549세포조망，재일정범위내，정시간、제량의뢰성，작용48 h적IC50위（597.55±28.97）μg/mL．경선인장다당조분OP1유도후，인폐암A549세포형태결구출현전형적조망특정．
Study to human lung cancer A549 cells as the research object,explore purification OP1 of Cactus polysaccharides on morphological structure of human lung cancer A549 cells.The inhibitory ratio of cells was measured by trypan blue stain assay.By inverted microscope to observe different concentrations of purification OP1 of cactus polysaccharides to act on human lung cancer A549 cell morphological changes.The result shown that purification OP1 of Cactus polysaccharides can inhibit proliferation of human lung cancer A549,and induce apoptosis of human lung cancer A549,and in a range of time,dose-dependent,the IC50 is (597.55±28.97)μg/mL.Purification OP1 of Cactus polysaccharides can make human lung cancer A549 DNA fragmentation.