山东医学高等专科学校学报
山東醫學高等專科學校學報
산동의학고등전과학교학보
JOURNAL OF SHANDONG MEDICAL COLLEGE
2014年
4期
267-268
,共2页
HPLC%杜仲双降袋泡茶%绿原酸%质量控制
HPLC%杜仲雙降袋泡茶%綠原痠%質量控製
HPLC%두중쌍강대포다%록원산%질량공제
HPLC%Eucommia bark tea in bag%Chlorogenic acid%Quality control
目的:采用高效液相色谱法(HPLC)建立杜仲双降袋泡茶的质量控制方法。方法采用C18(4.6mm ×150mm ,5μm)色谱柱;流动相为乙腈(A):0.8%冰醋酸溶液(B)=6:94;检测波长:327nm ;柱温:35℃;流速:1.0ml/min ;进样量:10μl;结果绿原酸在0.089~1.335μg范围内呈良好的线性关系,线性回归方程为:y =3×106x -37523,r =1。回收率为101.29%,RSD为0.88%( n =9)。结论该方法简便、快速、准确,能对杜仲双降袋泡茶中绿原酸成分有效地进行质量控制。
目的:採用高效液相色譜法(HPLC)建立杜仲雙降袋泡茶的質量控製方法。方法採用C18(4.6mm ×150mm ,5μm)色譜柱;流動相為乙腈(A):0.8%冰醋痠溶液(B)=6:94;檢測波長:327nm ;柱溫:35℃;流速:1.0ml/min ;進樣量:10μl;結果綠原痠在0.089~1.335μg範圍內呈良好的線性關繫,線性迴歸方程為:y =3×106x -37523,r =1。迴收率為101.29%,RSD為0.88%( n =9)。結論該方法簡便、快速、準確,能對杜仲雙降袋泡茶中綠原痠成分有效地進行質量控製。
목적:채용고효액상색보법(HPLC)건립두중쌍강대포다적질량공제방법。방법채용C18(4.6mm ×150mm ,5μm)색보주;류동상위을정(A):0.8%빙작산용액(B)=6:94;검측파장:327nm ;주온:35℃;류속:1.0ml/min ;진양량:10μl;결과록원산재0.089~1.335μg범위내정량호적선성관계,선성회귀방정위:y =3×106x -37523,r =1。회수솔위101.29%,RSD위0.88%( n =9)。결론해방법간편、쾌속、준학,능대두중쌍강대포다중록원산성분유효지진행질량공제。
Objective To use HPLC to establish a quality control method for the determination of chlorogenic acid in eucommia bark tea .Methods HPLC analysis was performed with C 18 chromatographic column (4 .6 × 150 mm ,5 μm) and a mobile phase of acetonitrile /0 .8% of acetic acid (6/94) .The detection wave length was 327 nm ,the column temperature was 35℃ and the flow rate was 1 .0 ml/min-1 .Results The linear regression equation was y =3 × 106x -37523 ( r = 1) ,linear range was 0 .089~1 .335 μg · mL-1 ,and the average recovery was 101 .29% ( n=9) with RSD being 0 .88 % .Conclusion This method is convenient and accurate ,which can be used for the quality control of chlorogenic acid in eucommia bark tea in bag .