浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
9期
773-776
,共4页
严永兴%梁丽贞%袁艳蓉%沈咏慧%陈涛%吴春丽%刘慧丽
嚴永興%樑麗貞%袁豔蓉%瀋詠慧%陳濤%吳春麗%劉慧麗
엄영흥%량려정%원염용%침영혜%진도%오춘려%류혜려
大鼠%脑出血%胰岛素样生长因子-1%细胞凋亡
大鼠%腦齣血%胰島素樣生長因子-1%細胞凋亡
대서%뇌출혈%이도소양생장인자-1%세포조망
rats%intracerebral hemorrhage%insulin-like growth factor-1%apoptosis
目的：观察脑出血模型大鼠脑组织胰岛素样生长因子-1（IGF-1）的表达及其对细胞凋亡的影响。方法随机将105只大鼠分为正常对照组、假手术组及脑出血组，应用立体定向技术，将自体未抗凝血注入大鼠基底节区制备脑出血模型；分别在造模后6、24、48、72h和1周将大鼠断头取脑制作标本，采用TUNEL染色检测脑组织细胞凋亡、荧光定量RT-PCR检测IGF-1 mRNA表达，免疫组化分析IGF-1表达。结果脑出血后6h血肿周围脑组织IGF-1及TUNEL染色阳性细胞表达升高，其中IGF-1在24h达高峰，48h开始下降，而细胞凋亡于72h达高峰，1周开始下降，表达均明显高于假手术组和正常对照组（P<0.05，P<0.01）；且IGF-1表达与细胞凋亡数呈正相关（P<0.05）。结论脑出血后IGF-1表达上调，参与了脑出血的病理生理过程，可能对脑出血继发神经损伤起重要的保护作用。
目的：觀察腦齣血模型大鼠腦組織胰島素樣生長因子-1（IGF-1）的錶達及其對細胞凋亡的影響。方法隨機將105隻大鼠分為正常對照組、假手術組及腦齣血組，應用立體定嚮技術，將自體未抗凝血註入大鼠基底節區製備腦齣血模型；分彆在造模後6、24、48、72h和1週將大鼠斷頭取腦製作標本，採用TUNEL染色檢測腦組織細胞凋亡、熒光定量RT-PCR檢測IGF-1 mRNA錶達，免疫組化分析IGF-1錶達。結果腦齣血後6h血腫週圍腦組織IGF-1及TUNEL染色暘性細胞錶達升高，其中IGF-1在24h達高峰，48h開始下降，而細胞凋亡于72h達高峰，1週開始下降，錶達均明顯高于假手術組和正常對照組（P<0.05，P<0.01）；且IGF-1錶達與細胞凋亡數呈正相關（P<0.05）。結論腦齣血後IGF-1錶達上調，參與瞭腦齣血的病理生理過程，可能對腦齣血繼髮神經損傷起重要的保護作用。
목적：관찰뇌출혈모형대서뇌조직이도소양생장인자-1（IGF-1）적표체급기대세포조망적영향。방법수궤장105지대서분위정상대조조、가수술조급뇌출혈조，응용입체정향기술，장자체미항응혈주입대서기저절구제비뇌출혈모형；분별재조모후6、24、48、72h화1주장대서단두취뇌제작표본，채용TUNEL염색검측뇌조직세포조망、형광정량RT-PCR검측IGF-1 mRNA표체，면역조화분석IGF-1표체。결과뇌출혈후6h혈종주위뇌조직IGF-1급TUNEL염색양성세포표체승고，기중IGF-1재24h체고봉，48h개시하강，이세포조망우72h체고봉，1주개시하강，표체균명현고우가수술조화정상대조조（P<0.05，P<0.01）；차IGF-1표체여세포조망수정정상관（P<0.05）。결론뇌출혈후IGF-1표체상조，삼여료뇌출혈적병리생리과정，가능대뇌출혈계발신경손상기중요적보호작용。
To investigate the expression of insulin-like growth factor-1 (IGF-1) in brain tissue of rats with experimental intracerebral hemorrhage and its effect on apoptosis. Methods One hundred and five rats were randomly divided into normal control group (n=5),sham-operated group (n=50),and intracerebral hemorrhage group (n=50). Intracerebral hemorrhage was induced by stereotaxic injection of autologous blood into right caudate nucleus of rats. Rats were sacrifice at 6,24,48,72h and 1 week after the establishment of animal models,respec-tively. TUNEL method was used to detect apoptosis. Both of RT-PCR and immunohistochemistry were used to de-tect the expression of IGF-1 in brain tissues. Results The expression of IGF-1 and TUNEL-positive cells in per-i-hematomal brain tissue started to increase at 6h;the expression of IGF-1 peaked at 24h and began to decrease at 48h;apoptosis peaked at 72h and began to decrease at 1 week. The expression of IGF-1 and TUNEL-positive cells were higher than those in sham-operated group and normal control group at each time point (P<0.05 or P<0.01). IGF-1 expression was positively related with apoptosis cells(P<0.05). Conclusion The expression of IGF-1 in brain tissue was up-regulated after intracerebral hemorrhage. It participates in the pathophysiologic course of in-tracerebral hemorrhage and has protective effect against secondary neuronal injury.
