黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2014年
9期
100-103
,共4页
中国红豆杉%紫杉醇%内生真菌%薄层层析%鉴定
中國紅豆杉%紫杉醇%內生真菌%薄層層析%鑒定
중국홍두삼%자삼순%내생진균%박층층석%감정
Taxus chinensis%taxol%endophyte fungi%thin layer chromatography%identification
为了开发生产紫杉醇的新途径,以中国红豆杉根、茎、叶为试验材料,对其中产紫杉醇的内生真菌进行分离和鉴定。结果表明:从中国红豆杉中共分离出10株内生真菌,在PDA液体培养基中发酵后经薄层层析法检测,出2株能够产生紫杉醇,对产紫杉醇的菌株进行形态学鉴定表明,其中一株为青霉属,另一株为芽枝霉属。
為瞭開髮生產紫杉醇的新途徑,以中國紅豆杉根、莖、葉為試驗材料,對其中產紫杉醇的內生真菌進行分離和鑒定。結果錶明:從中國紅豆杉中共分離齣10株內生真菌,在PDA液體培養基中髮酵後經薄層層析法檢測,齣2株能夠產生紫杉醇,對產紫杉醇的菌株進行形態學鑒定錶明,其中一株為青黴屬,另一株為芽枝黴屬。
위료개발생산자삼순적신도경,이중국홍두삼근、경、협위시험재료,대기중산자삼순적내생진균진행분리화감정。결과표명:종중국홍두삼중공분리출10주내생진균,재PDA액체배양기중발효후경박층층석법검측,출2주능구산생자삼순,대산자삼순적균주진행형태학감정표명,기중일주위청매속,령일주위아지매속。
In order to develop new ways of taxol production ,taking roots ,stems and leaves of Taxus chinensis as materials ,the taxol-producing endophytic fungi was isolated and identified .The results showed that ten strains of endophytic fungi had been isolated from Taxus chinensis ,two strains could produce taxol by thin layer chro-matography after fermentation in the PDA liquid culture medium ,through morphological identification ,it was determined that a strain was Penicillium ,another strain was Cladosporium .