CAJ | 학술논문

建立了采用固相萃取-超高液相色谱/紫外检测测定烟用水基胶中丙烯酰胺的分析方法。样品经超纯水进行超声提取，固相萃取柱进行富集净化后，经HSS T3色谱柱分离，以甲醇和水为流动相等度洗脱，紫外检测，外标法定量。结果显示，丙烯酰胺在浓度0．01~20．0μg·mL-1范围内有良好的线性关系，相关系数为0．9999。丙烯酰胺三个不同浓度的加标回收率为91．7%~105．1%；相对标准偏差为1．3%~4．7%( n=6)；检出限为3．5μg·L-1。该方法分析时间短、结果准确可靠，满足烟用水基胶中丙烯酰胺的测定。
건립료채용고상췌취-초고액상색보/자외검측측정연용수기효중병희선알적분석방법。양품경초순수진행초성제취，고상췌취주진행부집정화후，경HSS T3색보주분리，이갑순화수위류동상등도세탈，자외검측，외표법정량。결과현시，병희선알재농도0．01~20．0μg·mL-1범위내유량호적선성관계，상관계수위0．9999。병희선알삼개불동농도적가표회수솔위91．7%~105．1%；상대표준편차위1．3%~4．7%( n=6)；검출한위3．5μg·L-1。해방법분석시간단、결과준학가고，만족연용수기효중병희선알적측정。
A solid phase extraction( SPE)-ultra performance liquid chromatography( UPLC)/UV method was developed for the de-termination of acrylamide in water-based emulsion adhesives. The samples dispersed in water were extracted in an ultrasonic bath, and the extract was concentrated and purified with SPE cartridges. Chromatographic separation was performed on a HSS T3 column with isocratic elution using methanol and water. The target compound was determined and quantified by UPLC/UV. The linear re-gression analysis data for the calibration curve shows good linear relationship with correlation coefficient of 0. 9999 in the concentra-tion range of 0. 01-20. 0 μg·mL-1 . The average spiked recoveries of three different level of acrylamide are in the range of 91. 7%-105. 1%with the relative standard deviations(RSDs)between 1. 3%and 4. 7%(n=6). The limit of detection is 3. 5 μg·L-1. The method is accurate,simple,rapid,and suitable for the analysis of acrylamide in water-based emulsion adhesives for cigarette.