CAJ | 학술논문

利用正相和反相柱色谱,Sephadex LH-20凝胶柱色谱及反相HPLC等方法从昆虫共生真菌Paraconiothy-rium brasiliense的发酵产物中分离得到9个环二肽类化合物,经NMR,MS及理化性质等方法,结构确定为环(L-亮氨酸-L-酪氨酸)(1),环(L-色氨酸-L-脯氨酸)(2),环(L-脯氨酸-L-苯丙氨酸)(3),环(L-脯氨酸-L-酪氨酸)(4),环(L-脯氨酸-L-亮氨酸)(5),环(L-亮氨酸-反式-4-羟基-L-脯氨酸)(6),环(L-异亮氨酸-L-脯氨酸)(7),环(L-缬氨酸-L-脯氨酸)(8),环(L-亮氨酸-L-异亮氨酸)(9)。所有化合物均为首次从该属菌种中分离得到。
이용정상화반상주색보,Sephadex LH-20응효주색보급반상HPLC등방법종곤충공생진균Paraconiothy-rium brasiliense적발효산물중분리득도9개배이태류화합물,경NMR,MS급이화성질등방법,결구학정위배(L-량안산-L-락안산)(1),배(L-색안산-L-포안산)(2),배(L-포안산-L-분병안산)(3),배(L-포안산-L-락안산)(4),배(L-포안산-L-량안산)(5),배(L-량안산-반식-4-간기-L-포안산)(6),배(L-이량안산-L-포안산)(7),배(L-힐안산-L-포안산)(8),배(L-량안산-L-이량안산)(9)。소유화합물균위수차종해속균충중분리득도。
Nine cyclic dipeptides secondary metabolites were isolated from the symbiotic fungus paraconiothyrium brasiliense, by normal phase silica gel,Sephadex LH-20 chromatography and reverse phase HPLC. Their structures were elucidated as cyclo-( L-Leu-L-Tyr)(1),cyclo-(L-Trp-L-Pro)(2),cyclo-(L-Pro-L-Phe)(3),cyclo-(L-Pro-L-Tyr)(4),cyclo-(L-Pro-L-Leu)(5),cyclo-(L-Leu-trans-4-hydroxy-L-Pro)(6),cyclo-(L-Ile-L-Pro)(7),cyclo-(L-Val-L-Pro)(8),cyclo-(L-Leu-L-Ile)(9)by NMR,MS and physicochemical properties. All the compounds were isolated from the symbiotic fungus paraconiothyrium brasiliense for the first time.