CAJ | 학술논문

采集50例乙型肝炎未并发消化道出血患者的血液和粪便标本,采用酶联免疫法检测粪便和血清中乙肝病毒标志物( HBVM ), QIAmp DNA Stool kit 试剂盒法提取粪便总DNA,巢式 PCR 定性检测粪便中乙肝病毒 DNA ( HBV-DNA),实时荧光定量PCR( FQ-PCR)定量检测血清和粪便中HBV-DNA。根据血清HBVM 阳性的不同组合(感染模式)将50例患者分成3组,粪便乙肝表面抗原( HBsAg)阳性37例,血清HBsAg阳性46例,两者检出率差异有统计学意义(P<0.05),1例血清阴性者,粪便中HBsAg阳性。 FQ-PCR定量检测血清和粪便HBV-DNA含量分别为4.35±1.49和4.50±1.59。血清HBV-DNA检测,30例阳性(60%),粪便中HBV-DNA共检测出31例阳性(62%)。
채집50례을형간염미병발소화도출혈환자적혈액화분편표본,채용매련면역법검측분편화혈청중을간병독표지물( HBVM ), QIAmp DNA Stool kit 시제합법제취분편총DNA,소식 PCR 정성검측분편중을간병독 DNA ( HBV-DNA),실시형광정량PCR( FQ-PCR)정량검측혈청화분편중HBV-DNA。근거혈청HBVM 양성적불동조합(감염모식)장50례환자분성3조,분편을간표면항원( HBsAg)양성37례,혈청HBsAg양성46례,량자검출솔차이유통계학의의(P<0.05),1례혈청음성자,분편중HBsAg양성。 FQ-PCR정량검측혈청화분편HBV-DNA함량분별위4.35±1.49화4.50±1.59。혈청HBV-DNA검측,30례양성(60%),분편중HBV-DNA공검측출31례양성(62%)。
We collected the samples offeces and serum for all patients. HBVM in serum and feces were determined with ELISA. Using QIAmp DNA Stool kit to extract human fecal DNA. FQ-PCR were utilized for analysis the content of HBV-DNA in feces and serum while it was detected qualitatively by nested-PCR. Depending on the combination of HBVM, 50 patients were divided into three groups. Hepatitis B surface antigen was positive in 37 cases of stool and 46 cases of serum, both the detection rates were significant ( P <0.05 ) . FQ-PCR quantitative detection of HBV-DNA in serum and fecal contents were 4.35 ±1.49 and 4.50 ±1.59 . The positive rates of HBsAg were 74%, 92% in feces and serum, respectively. HBsAg was found in feces in one patient without HBsAg in serum. In 50 patients,the positive rates of HBV-DNA were 62%, 60% in feces and serum,respectively.