安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
10期
1422-1427
,共6页
陈琪%步仰高%王杨%陈志武
陳琪%步仰高%王楊%陳誌武
진기%보앙고%왕양%진지무
缺氧缺血%脑%胰岛素样生长因子-1%细胞色素C%caspase-3%小鼠%新生
缺氧缺血%腦%胰島素樣生長因子-1%細胞色素C%caspase-3%小鼠%新生
결양결혈%뇌%이도소양생장인자-1%세포색소C%caspase-3%소서%신생
hypoxia-ischemia%cerebral%insulin-like growth factor-1%cytochrome C%caspase-3%mouse%neonatal
目的探讨胰岛素样生长因子-1( IGF-1)对缺氧缺血性脑损伤( HIBD)新生小鼠脑细胞线粒体细胞色素C( Cyt-C)、caspase-3表达的影响。方法108只新生C57/BL小鼠随机分为正常对照组、缺氧缺血( HI)组和HI+IGF-1组,改良Rice法制作新生小鼠右脑 HIBD模型。 HI+IGF-1组于HI后即刻予腹腔注射IGF-1(50μg/kg),HI组及正常对照组注射等量的生理盐水。应用Western blot和RT-PCR法检测各组皮质、海马、丘脑0、3、6、12、24和48 h Cyt-C、caspase-3蛋白和mRNA表达。结果① HI组Cyt-C、caspase-3 mR-NA及蛋白表达明显增加出现在皮质术后3 h,海马术后6 h,丘脑出现在术后12 h,明显迟于皮质及海马；② HI+IGF-1组Cyt-C、caspase-3 mRNA及蛋白表达在HI组表达明显增加的相应时间点虽较正常对照组升高,但较HI组表达明显降低,3组之间比较差异有统计学意义( P<0.05)；③ HI组皮质Cyt-C、caspase-3 mRNA及蛋白表达于术后3 h表达明显增加,6 h有所下降,12~24 h达到另一高峰,峰值时间点HI+IGF-1组的表达较HI组均降低(P<0.05)。结论 IGF-1可能通过抑制Cyt-C释放,减少caspase-3表达,发挥对新生小鼠HIBD的神经保护作用,IGF-1对皮质的保护有两个时间点。
目的探討胰島素樣生長因子-1( IGF-1)對缺氧缺血性腦損傷( HIBD)新生小鼠腦細胞線粒體細胞色素C( Cyt-C)、caspase-3錶達的影響。方法108隻新生C57/BL小鼠隨機分為正常對照組、缺氧缺血( HI)組和HI+IGF-1組,改良Rice法製作新生小鼠右腦 HIBD模型。 HI+IGF-1組于HI後即刻予腹腔註射IGF-1(50μg/kg),HI組及正常對照組註射等量的生理鹽水。應用Western blot和RT-PCR法檢測各組皮質、海馬、丘腦0、3、6、12、24和48 h Cyt-C、caspase-3蛋白和mRNA錶達。結果① HI組Cyt-C、caspase-3 mR-NA及蛋白錶達明顯增加齣現在皮質術後3 h,海馬術後6 h,丘腦齣現在術後12 h,明顯遲于皮質及海馬；② HI+IGF-1組Cyt-C、caspase-3 mRNA及蛋白錶達在HI組錶達明顯增加的相應時間點雖較正常對照組升高,但較HI組錶達明顯降低,3組之間比較差異有統計學意義( P<0.05)；③ HI組皮質Cyt-C、caspase-3 mRNA及蛋白錶達于術後3 h錶達明顯增加,6 h有所下降,12~24 h達到另一高峰,峰值時間點HI+IGF-1組的錶達較HI組均降低(P<0.05)。結論 IGF-1可能通過抑製Cyt-C釋放,減少caspase-3錶達,髮揮對新生小鼠HIBD的神經保護作用,IGF-1對皮質的保護有兩箇時間點。
목적탐토이도소양생장인자-1( IGF-1)대결양결혈성뇌손상( HIBD)신생소서뇌세포선립체세포색소C( Cyt-C)、caspase-3표체적영향。방법108지신생C57/BL소서수궤분위정상대조조、결양결혈( HI)조화HI+IGF-1조,개량Rice법제작신생소서우뇌 HIBD모형。 HI+IGF-1조우HI후즉각여복강주사IGF-1(50μg/kg),HI조급정상대조조주사등량적생리염수。응용Western blot화RT-PCR법검측각조피질、해마、구뇌0、3、6、12、24화48 h Cyt-C、caspase-3단백화mRNA표체。결과① HI조Cyt-C、caspase-3 mR-NA급단백표체명현증가출현재피질술후3 h,해마술후6 h,구뇌출현재술후12 h,명현지우피질급해마；② HI+IGF-1조Cyt-C、caspase-3 mRNA급단백표체재HI조표체명현증가적상응시간점수교정상대조조승고,단교HI조표체명현강저,3조지간비교차이유통계학의의( P<0.05)；③ HI조피질Cyt-C、caspase-3 mRNA급단백표체우술후3 h표체명현증가,6 h유소하강,12~24 h체도령일고봉,봉치시간점HI+IGF-1조적표체교HI조균강저(P<0.05)。결론 IGF-1가능통과억제Cyt-C석방,감소caspase-3표체,발휘대신생소서HIBD적신경보호작용,IGF-1대피질적보호유량개시간점。
Objective To discuss the effect of IGF-1 on the expression of cytochrome C ( Cyt-C) and caspase-3 in neonatal mice with hypoxic-ischemic brain damage ( HIBD) . Methods One hundred and eight C57/BL neonatal mice were randomly divided into normal control group ( n=36 ) and HI group ( n=36 ) and HI+IGF-1 group ( n=36 ) . Mouse HIBD model was prepared according to the Rice-Vannucci method. HI+IGF-1 group was immediately injected with 50 μg/kg IGF-1 after HI;HI group and normal control group were injected with the same amount of saline solution. RT-PCR and Western blot methods were used to measure the mRNA and protein expression of Cyt-C and caspase-3 0, 3, 6, 12, 24 and 48 h after HI. Results ①In HI group, the increase of Cyt-C and caspase-3 mRNA and protein expression intensity appeared at 3 h in cerebral cortex, at 6 h in hippocampus and at 12 h in thalamus, delayed compared with cortex and hippocampus; ② HI group Cyt-C and caspase-3 mRNA and protein expression increased compared with those in the normal control groups at the same point, and those in the HI+IGF-1 group also increased compared with that in the normal control groups, but decreased compared with that in the HI group. There was statistical significance among the groups (P<0.05);③The Cyt-C and caspase-3 mRNA and protein expression of Cortex significantly increased after 3 hours, and then decreased after 6 hours, and reached another peak value in 12~24 hours. The expressions of Cyt-C and caspase-3 mRNA in HI+IGF-1 group were lower in the two peak time than HI group (P<0.05). Conclusion IGF-1 may inhibit the release of Cyt-C, and reduce the expression of caspase-3, and provide neuroprotection against HIBD in neonatal mice. IGF-1 has two time points to protect the cortex.
