CAJ | 학술논문

目的研究橙皮苷衍生物Y-12( HY-12)对免疫低下小鼠的免疫功能的影响。方法以连续2d腹腔注射给药环磷酰胺(50 mg/kg),建立小鼠免疫低下模型,同时分别用IgG和IgM测量试验、巨噬细胞吞噬碳试验和迟发性超敏反应,观测HY-12不同浓度对免疫低下小鼠免疫系统的影响。结果巨噬细胞吞噬碳试验中,环磷酰胺诱免疫低下模型小鼠的廓清指数( K )和巨噬细胞的吞噬指数(α)能被HY-12(100、200、400 mg/kg)组增加；溶血素试验中,HY-12(200、400 mg/kg)组不仅能增多免疫低下模型小鼠血清中IgG和IgM,同时脾细胞溶血素也被增多；HY-12(200、400 mg/kg)组可明显有助于免疫低下模型小鼠DTH 反应；并能提高脾淋巴细胞IL-22含量；HY-12(100、200、400 mg/kg)能提高CD4+、CD8+细胞数以及 CD4+/CD8+比值。结论 HY-12能普遍增强模型小鼠的免疫系统反应,对免疫低下模型小鼠的免疫功能有很好的提高作用。
목적연구등피감연생물Y-12( HY-12)대면역저하소서적면역공능적영향。방법이련속2d복강주사급약배린선알(50 mg/kg),건립소서면역저하모형,동시분별용IgG화IgM측량시험、거서세포탄서탄시험화지발성초민반응,관측HY-12불동농도대면역저하소서면역계통적영향。결과거서세포탄서탄시험중,배린선알유면역저하모형소서적곽청지수( K )화거서세포적탄서지수(α)능피HY-12(100、200、400 mg/kg)조증가；용혈소시험중,HY-12(200、400 mg/kg)조불부능증다면역저하모형소서혈청중IgG화IgM,동시비세포용혈소야피증다；HY-12(200、400 mg/kg)조가명현유조우면역저하모형소서DTH 반응；병능제고비림파세포IL-22함량；HY-12(100、200、400 mg/kg)능제고CD4+、CD8+세포수이급 CD4+/CD8+비치。결론 HY-12능보편증강모형소서적면역계통반응,대면역저하모형소서적면역공능유흔호적제고작용。
Objective To study the immunomodulatory activity of HY-12 on immunosuppressive mice. MethodsCy (50 mg/kg) was administered by intraperitoneal ( ip) injection for 2 consecutive days to induce immunosup-pressive model. Carbon clearance, quantitative hemolysis and DNFB-induced delayed-type hypersensitivity were ap-plied to assay effects of HY-12 on nonspecific immunity, humoral immunity and cellular immunity. Results In car-bon clearance test, the clearance index (K) and values of phagocytic index (α) were elevated by HY-12 (100, 200, 400 mg/kg), indicating the phagocytosis of macrophages was enhanced in immunosuppressive mice. In quan-titative hemolysis, productions of IgG and IgM and hemolysin were enhanced by HY-12 (200, 400 mg/kg). HY-12 (200, 400 mg/kg) obviously increased DTH reactivity and enhanced expression of IL-22 in immunosuppressive mice. HY-12 (100, 200, 400 mg/kg) not only increased expression of CD4 + and CD8 +, but also enhanced the ratio of the two. Conclusion HY-12 shows significant immunomodulatory property on immunosuppressive mice through specific and nonspecific immunity.