CAJ | 학술논문

目的探讨丹皮酚在体外对人食管癌Eca-109细胞放射增敏作用的机制。方法采用 MTT 法检测丹皮酚对Eca-109细胞的增殖抑制作用。松胞素B ( CB )微核法研究丹皮酚对Eca-109放射敏感性的影响。克隆形成试验观察丹皮酚对Eca-109细胞的放射增敏作用。免疫组织化学法分析射线联合丹皮酚对Eca-109细胞的COX-2和Survivin表达的影响。结果①丹皮酚对人食管癌细胞Eca-109有生长抑制作用,半数抑制浓度( IC50)为(59.40±2.23) mg/L。②7.81 mg/L 丹皮酚加重照射后Eca-109细胞的染色质损伤,提高其放射敏感性。③克隆形成实验显示,丹皮酚对Eca-109细胞有增敏作用,放射增敏比为1.092。④6 Gy X射线照射后48 h,Eca-109细胞中的COX-2和Survivin的表达增加(P<0.01),3.91、7.81、15.63 mg/L丹皮酚可不同程度降低照射后COX-2和Survivin的表达,其中3.91 mg/L丹皮酚对COX-2表达的下调差异无统计学意义,其余差异均有统计学意义( P<0.01)。两种蛋白表达的变化呈正相关性(r=0.955,P<0.05)。结论丹皮酚在体外对人食管癌细胞 Eca-109有放射增敏作用,其作用机制可能与下调COX-2和Survivin两种蛋白的表达相关。
목적탐토단피분재체외대인식관암Eca-109세포방사증민작용적궤제。방법채용 MTT 법검측단피분대Eca-109세포적증식억제작용。송포소B ( CB )미핵법연구단피분대Eca-109방사민감성적영향。극륭형성시험관찰단피분대Eca-109세포적방사증민작용。면역조직화학법분석사선연합단피분대Eca-109세포적COX-2화Survivin표체적영향。결과①단피분대인식관암세포Eca-109유생장억제작용,반수억제농도( IC50)위(59.40±2.23) mg/L。②7.81 mg/L 단피분가중조사후Eca-109세포적염색질손상,제고기방사민감성。③극륭형성실험현시,단피분대Eca-109세포유증민작용,방사증민비위1.092。④6 Gy X사선조사후48 h,Eca-109세포중적COX-2화Survivin적표체증가(P<0.01),3.91、7.81、15.63 mg/L단피분가불동정도강저조사후COX-2화Survivin적표체,기중3.91 mg/L단피분대COX-2표체적하조차이무통계학의의,기여차이균유통계학의의( P<0.01)。량충단백표체적변화정정상관성(r=0.955,P<0.05)。결론단피분재체외대인식관암세포 Eca-109유방사증민작용,기작용궤제가능여하조COX-2화Survivin량충단백적표체상관。
Objective To investigate the radiosensitization effect and underlying mechanism of Paeonol on human esophageal squamous carcinoma cell line Eca-109 in vitro. Methods Cytotoxic effect of Paeonol on cells was eval-uated by MTT assay. Radiosensitivity was tested by cytokinesis-block micronucleus assay. Cell survival was evalua-ted by Clonogenic assay. The expression of COX-2 and Survivin were detected by immunohistochemical method. Re-sults ① Cell growth was inhibited by Paeonol in a dose-dependent manner and the IC50 was (59.40±2.23) mg/L. ② Paeonol combined with irradiation increased chromatin damage of Eca-109 cell, which meat enhancement of radiosensitivity. ③ Paeonol could enhance cell radiosensitivity and the sensitizing enhancement ratio ( SER) was 1. 092.④ After exposure to 6 Gy X-ray for 48 h, the protein expression of COX-2 and Survivin was up-regulation (P<0.01). On the contrary, pretreatment (using 3. 91,7. 81,15. 63 mg/L Paeonol) combined with radiation re-duced the protein expression of COX-2 and Survivin in varying degrees, showing significant differences(P<0.01), except the protein expression of COX-2 at 3. 91 mg/L. The down-regulation of the two protein expression was posi-tively correlated(r=0.955,P<0. 05). Conclusion Paeonol improves radiosensitivity of human esophageal carci-noma cell line Eca-109 in vitro, perhaps by inhibiting the expression of COX-2 and Survivin.