安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
10期
1409-1412,1413
,共5页
急性单核细胞白血病细胞株SHI-1%肿瘤%VOCs%固相微萃取-气相色谱/质谱技术
急性單覈細胞白血病細胞株SHI-1%腫瘤%VOCs%固相微萃取-氣相色譜/質譜技術
급성단핵세포백혈병세포주SHI-1%종류%VOCs%고상미췌취-기상색보/질보기술
acute mononuclear leukemia cells SHI-1%tumors%VOCs%SPME-GC/MS
目的检测急性单核细胞白血病细胞株SHI-1培养瓶顶空气体中挥发性有机物( VOCs),分析该细胞对其组分的影响,并筛选出白血病细胞株SHI-1代谢产物中具有特征性的VOCs,探讨VOCs在白血病患者缓解后随访或与其他肿瘤鉴别诊断中的价值。方法收集急性单核细胞白血病细胞株SHI-1、人巨噬细胞株和空白培养液培养瓶顶空气体中的VOCs,采用固相微萃取-气相色谱/质谱技术( SPME-GC/MS)对其进行检测,应用双边Mann-Whitney U检验分析两两之间的差异性,筛选出与 SHI-1细胞有关的特征性VOCs。结果 SHI-1细胞培养瓶顶空气体中可检测出8种特征性挥发性有机物,分别为2,4-二甲基庚烷、苯、4-甲基辛烷、氯仿、3,7-二甲基十二烷、己醇、环己醇、十六烷,其中烷烃类、苯类物质可检测量较对照组中明显增高,醇类明显减少。结论 SHI-1细胞可引起培养瓶顶空气体中VOCs组分的改变,其中多种烷烃类物质、苯含量增多,醇类含量减少,提示上述物质有可能作为白血病细胞特征性标志物。
目的檢測急性單覈細胞白血病細胞株SHI-1培養瓶頂空氣體中揮髮性有機物( VOCs),分析該細胞對其組分的影響,併篩選齣白血病細胞株SHI-1代謝產物中具有特徵性的VOCs,探討VOCs在白血病患者緩解後隨訪或與其他腫瘤鑒彆診斷中的價值。方法收集急性單覈細胞白血病細胞株SHI-1、人巨噬細胞株和空白培養液培養瓶頂空氣體中的VOCs,採用固相微萃取-氣相色譜/質譜技術( SPME-GC/MS)對其進行檢測,應用雙邊Mann-Whitney U檢驗分析兩兩之間的差異性,篩選齣與 SHI-1細胞有關的特徵性VOCs。結果 SHI-1細胞培養瓶頂空氣體中可檢測齣8種特徵性揮髮性有機物,分彆為2,4-二甲基庚烷、苯、4-甲基辛烷、氯倣、3,7-二甲基十二烷、己醇、環己醇、十六烷,其中烷烴類、苯類物質可檢測量較對照組中明顯增高,醇類明顯減少。結論 SHI-1細胞可引起培養瓶頂空氣體中VOCs組分的改變,其中多種烷烴類物質、苯含量增多,醇類含量減少,提示上述物質有可能作為白血病細胞特徵性標誌物。
목적검측급성단핵세포백혈병세포주SHI-1배양병정공기체중휘발성유궤물( VOCs),분석해세포대기조분적영향,병사선출백혈병세포주SHI-1대사산물중구유특정성적VOCs,탐토VOCs재백혈병환자완해후수방혹여기타종류감별진단중적개치。방법수집급성단핵세포백혈병세포주SHI-1、인거서세포주화공백배양액배양병정공기체중적VOCs,채용고상미췌취-기상색보/질보기술( SPME-GC/MS)대기진행검측,응용쌍변Mann-Whitney U검험분석량량지간적차이성,사선출여 SHI-1세포유관적특정성VOCs。결과 SHI-1세포배양병정공기체중가검측출8충특정성휘발성유궤물,분별위2,4-이갑기경완、분、4-갑기신완、록방、3,7-이갑기십이완、기순、배기순、십륙완,기중완경류、분류물질가검측량교대조조중명현증고,순류명현감소。결론 SHI-1세포가인기배양병정공기체중VOCs조분적개변,기중다충완경류물질、분함량증다,순류함량감소,제시상술물질유가능작위백혈병세포특정성표지물。
Objective To find out the impact of SHI-1 cells on VOCs in the headspace, and to assess the feasibili-ty of VOCs analysis in leukemia patients or with other tumors in the differential diagnosis by determing VOCs in the headspace of acute mononuclear leukemia cells(SHI-1). Methods The air samples from the headspace of SHI-1, Human macrophages cells and medium control were collected by syringes, and then determined by means of solid phase microextraction-gas chromatography/mass spectrometry ( SPME-GC/MS ) in order to have a better under-standing of the concentration distributions and changes of VOCs in SHI-1 cells' headspace. Results Using Mann-Whitney U test, we found that eight kinds of VOCs were different among them. They were 2,4-dimethyl heptanes, 4-methyl octane, chloroform, benzene, 3,7-dimethyl dodecanese, hexanol, cyclohexanol, cetane. And alkane, benzene were significantly higher than the control group ( P<0.05 ) , alcohol decreased significantly ( P<0.05 ) . Conclusion SHI-1 cells have an impact on the concentration distributions of VOCs in their headspace. The most outstanding VOCs are alkanes, alcohol and benzene compared with the blank control group. They may be potential markers characteristic of leukemia cells.
