北京医学
北京醫學
북경의학
BEIJING MEDICAL JOURNAL
2014年
9期
752-755
,共4页
孙斌%李雯%计云霞%郭向华%杨晓珍%郝美君%李伟华%白飞云%王延军%郑加生%陈德喜
孫斌%李雯%計雲霞%郭嚮華%楊曉珍%郝美君%李偉華%白飛雲%王延軍%鄭加生%陳德喜
손빈%리문%계운하%곽향화%양효진%학미군%리위화%백비운%왕연군%정가생%진덕희
磷脂酰肌醇蛋白聚糖3%人类白细胞抗原%T淋巴细胞%表位肽
燐脂酰肌醇蛋白聚糖3%人類白細胞抗原%T淋巴細胞%錶位肽
린지선기순단백취당3%인류백세포항원%T림파세포%표위태
Glypican-3( GPC3)%HLA%Tlymphocyte%Epitope
目的：设计合成人类白细胞抗原（HLA）-A11限制性T细胞识别的磷脂酰肌醇蛋白聚糖3（GPC3）多肽，并检测其免疫原性和反应性。方法利用BIMAS 和SYFPEITHI评分系统评价GPC3多肽与HLA-A11分子的结合能力，预测出相应的抗原谱，合成HLA-A1101限制性GPC3多肽库；流式细胞仪检测HCC患者HLA-A11分子的表达；多肽刺激外周血淋巴细胞，酶联免疫斑点试验（ELISPOT）检测T细胞对GPC3多肽的反应性。结果流式细胞仪检测32例HCC患者，7例HLA-A11分子表达阳性（21.9%）；ELISPOT检测发现17例（53.1%）HCC患者的T细胞应答阳性，7例（21.9%）HLA-A11表型检测阳性的 HCC患者，T细胞应答结果成强阳性。HLA-A11限制性GPC3多肽应答率符合人群中相应基因位点的自然表达率。结论 GPC3多肽设计合理，具有良好的免疫原性和反应性。
目的：設計閤成人類白細胞抗原（HLA）-A11限製性T細胞識彆的燐脂酰肌醇蛋白聚糖3（GPC3）多肽，併檢測其免疫原性和反應性。方法利用BIMAS 和SYFPEITHI評分繫統評價GPC3多肽與HLA-A11分子的結閤能力，預測齣相應的抗原譜，閤成HLA-A1101限製性GPC3多肽庫；流式細胞儀檢測HCC患者HLA-A11分子的錶達；多肽刺激外週血淋巴細胞，酶聯免疫斑點試驗（ELISPOT）檢測T細胞對GPC3多肽的反應性。結果流式細胞儀檢測32例HCC患者，7例HLA-A11分子錶達暘性（21.9%）；ELISPOT檢測髮現17例（53.1%）HCC患者的T細胞應答暘性，7例（21.9%）HLA-A11錶型檢測暘性的 HCC患者，T細胞應答結果成彊暘性。HLA-A11限製性GPC3多肽應答率符閤人群中相應基因位點的自然錶達率。結論 GPC3多肽設計閤理，具有良好的免疫原性和反應性。
목적：설계합성인류백세포항원（HLA）-A11한제성T세포식별적린지선기순단백취당3（GPC3）다태，병검측기면역원성화반응성。방법이용BIMAS 화SYFPEITHI평분계통평개GPC3다태여HLA-A11분자적결합능력，예측출상응적항원보，합성HLA-A1101한제성GPC3다태고；류식세포의검측HCC환자HLA-A11분자적표체；다태자격외주혈림파세포，매련면역반점시험（ELISPOT）검측T세포대GPC3다태적반응성。결과류식세포의검측32례HCC환자，7례HLA-A11분자표체양성（21.9%）；ELISPOT검측발현17례（53.1%）HCC환자적T세포응답양성，7례（21.9%）HLA-A11표형검측양성적 HCC환자，T세포응답결과성강양성。HLA-A11한제성GPC3다태응답솔부합인군중상응기인위점적자연표체솔。결론 GPC3다태설계합리，구유량호적면역원성화반응성。
Objective To design and synthesize GPC3 peptides recognized by HLA -A11 - restricted T lymphocytes, and to detect their immunogenicity and reactivity. Methods The GPC3 peptides and HLA-A11 molecules were evaluated, and the corresponding antigen spectrum was predicted by BIMAS and SYFPEITHI scoring system. HLA-A1101 which restricted GPC3 peptide library was synthesized; the expression of HLA-A11 molecules in hepatocell-lular carcinoma (HCC) patients was tested by flow cytometry; peripheral blood lymphocytes were stimulated with peptides, and reactive T lymphocytes were detected with ELISPOT for secretion of IFN-γ. Results Thirty-two HCC patients were detected by flow cytometry for HLA-A11 molecular expression with 7 cases (21.9%) positive. By ELISPOT, 17 cases were detected for T lymphocytes respose with positive. Meanwhile, the T lymphocytes responses in the 7 HCC patients were de-tected as strongly positive, showing that the HLA-A11 restricted GPC3 peptide response rate was consisted with the natural expression rate of the corresponding gene locus. Conclusion The GPC3 polypeptide is reasonable designed with good im-munogenicity and reactivity.
