CAJ | 학술논문

采用实时荧光定量PCR技术对132例急诊重症监扩室( EICU)患者血浆DNA水平进行定量检测，根据预后将患者分为存活组(95例)、死亡组(37例)，分别检测患者入EICU后即刻、24 h、48 h及1周血浆DNA的量；另选30例健康体检者为健康对照组。与健康对照组相比， EICU患者血浆DNA水平明显升高，各时相点血浆DNA水平升高差异均有统计学意义(P<0.05)。 EICU患者死亡组血浆DNA水平高于存活组，其中24 h、48 h时相点血浆DNA水平升高差异有统计学意义(P<0.05)。血浆DNA的受试者工作特征曲线(ROC)曲线下面积(AUC)较乳酸及 APACHEⅡ评分的AUC大，其中48 h血浆DNA的AUC最大为0.847(95% CI 0.648~1.000)。
채용실시형광정량PCR기술대132례급진중증감확실( EICU)환자혈장DNA수평진행정량검측，근거예후장환자분위존활조(95례)、사망조(37례)，분별검측환자입EICU후즉각、24 h、48 h급1주혈장DNA적량；령선30례건강체검자위건강대조조。여건강대조조상비， EICU환자혈장DNA수평명현승고，각시상점혈장DNA수평승고차이균유통계학의의(P<0.05)。 EICU환자사망조혈장DNA수평고우존활조，기중24 h、48 h시상점혈장DNA수평승고차이유통계학의의(P<0.05)。혈장DNA적수시자공작특정곡선(ROC)곡선하면적(AUC)교유산급 APACHEⅡ평분적AUC대，기중48 h혈장DNA적AUC최대위0.847(95% CI 0.648~1.000)。
The cell-free plasma DNA of the 132 critically ill patients admitted to the emergency intensive care unit ( EICU) was measured by real-time quantitative polymerase chain reaction assay for the humanβ-globin gene. Ac-cording to the prognosis, the patients were divided into survival group (95 cases) and death group (37 cases). Blood samples were collected after EICU admission, 24 hours after admission, 48 hours after admission, and 1 week after admission. 30 age-mached controls were collected from healthy volunteers healthy care centre. Com-pared with control group, at each time point, cell-free plasma DNA concentration was significantly higher in EICU patients (P<0. 05). Cell-free plasma DNA concentrations were higher in hospital non-survivors than in survivors, of which plasma DNA concentration of 24 hours after admission and 48 hours after admission was statistically signifi-cant ( P<0 . 05 ) . The area under the ROC curves ( AUC ) for plasma DNA was bigger than the lactate and A-PACHE IIscore, the maximum of AUC was 0. 847(95% CI 0.648~1.000) at the point of 48 hours after admis-sion.