CAJ | 학술논문

以肺腺癌A549细胞为筛选靶标，人胚肺二倍体helf细胞作反相吸附细胞，从噬菌体随机十二肽库中筛选出与肺癌细胞特异性结合的噬菌体多肽，用其替代一抗，酶标抗噬菌体抗体作二抗，建立检测肺癌细胞的免疫酶染色法。对噬菌体随机十二肽库进行4轮筛选和ELISA鉴定后，用亲和力和特异性最好的5号噬菌体克隆建立了检测肺癌细胞的免疫酶法。
이폐선암A549세포위사선파표，인배폐이배체helf세포작반상흡부세포，종서균체수궤십이태고중사선출여폐암세포특이성결합적서균체다태，용기체대일항，매표항서균체항체작이항，건립검측폐암세포적면역매염색법。대서균체수궤십이태고진행4륜사선화ELISA감정후，용친화력화특이성최호적5호서균체극륭건립료검측폐암세포적면역매법。
With A549 cells serving as target cells and helf cells as absorber cells, polypeptides specifically binding to lung cancer cells were screened out from Ph. D. -12TM Phage Display Peptide Library. Establish the immu-noenzymatic assay ( IEA) with the screened polypeptides replacing primary antibody, and the enzyme-marked anti phage antibody working as secondary antibody. After four rounds of panning and ELISA identification, the phage clone with best affinity and specificity to A549 cells was applied in the new method.