安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
9期
1320-1324
,共5页
冷再君%徐傲%徐修才%徐飞%伍权%操乐杰
冷再君%徐傲%徐脩纔%徐飛%伍權%操樂傑
랭재군%서오%서수재%서비%오권%조악걸
非小细胞肺癌%表皮生长因子受体%血浆
非小細胞肺癌%錶皮生長因子受體%血漿
비소세포폐암%표피생장인자수체%혈장
NSCLC%EGFR%plasma
目的多途径标本检测进展期非小细胞肺癌( aN-SCLC)患者表皮生长因子受体( EGFR)突变,及突变状态与患者临床病理学特征、标本来源及血清癌胚抗原( CEA)的关系。方法收集aNSCLC患者组织、细胞学及血浆标本,采用QIAGEN 公司新推出的 QIAamp Circulating Nucleic Acid Kit提取血浆循环游离DNA(cfDNA),使用蝎形探针扩增阻滞突变系统( sARMS)对提取DNA进行EGFR突变检测。结果103例aNSCLC患者组织(或细胞)学标本检测EGFR突变率为48.5%(50/103),与吸烟史、病理类型相关( P <0.05);与性别、年龄、身体状态评分、分期、血清 CEA 值无关;转移灶组突变率(58.6%)较原发灶组(35.6%)高,含有恶性胸腔积液(MPE)患者EGFR突变率(67.5%)较无MPE者(36.5%)高(P<0.05);29例血浆与组织(或细胞)学标本配对检测中,血浆阳性率为37.9%(11/29),敏感性为43.7%(7/16),特异性为69.2%(9/13)。结论 aNSCLC患者EGFR突变在非吸烟、腺癌人群中高发,与性别、年龄、血CEA、分期、身体状态无关。转移灶EGFR突变率较原发灶高,并发MPE 患者EGFR 突变率较无MPE 者高。使用 QIAamp Circulating Nucleic Acid Kit 提取血浆cfDNA 检测 EGFR 突变敏感性尚可,如降低成本,可作为组织标本的补充。由于肿瘤异质性的存在,应尽可能获取不同部位的肿瘤组织用于检测。
目的多途徑標本檢測進展期非小細胞肺癌( aN-SCLC)患者錶皮生長因子受體( EGFR)突變,及突變狀態與患者臨床病理學特徵、標本來源及血清癌胚抗原( CEA)的關繫。方法收集aNSCLC患者組織、細胞學及血漿標本,採用QIAGEN 公司新推齣的 QIAamp Circulating Nucleic Acid Kit提取血漿循環遊離DNA(cfDNA),使用蝎形探針擴增阻滯突變繫統( sARMS)對提取DNA進行EGFR突變檢測。結果103例aNSCLC患者組織(或細胞)學標本檢測EGFR突變率為48.5%(50/103),與吸煙史、病理類型相關( P <0.05);與性彆、年齡、身體狀態評分、分期、血清 CEA 值無關;轉移竈組突變率(58.6%)較原髮竈組(35.6%)高,含有噁性胸腔積液(MPE)患者EGFR突變率(67.5%)較無MPE者(36.5%)高(P<0.05);29例血漿與組織(或細胞)學標本配對檢測中,血漿暘性率為37.9%(11/29),敏感性為43.7%(7/16),特異性為69.2%(9/13)。結論 aNSCLC患者EGFR突變在非吸煙、腺癌人群中高髮,與性彆、年齡、血CEA、分期、身體狀態無關。轉移竈EGFR突變率較原髮竈高,併髮MPE 患者EGFR 突變率較無MPE 者高。使用 QIAamp Circulating Nucleic Acid Kit 提取血漿cfDNA 檢測 EGFR 突變敏感性尚可,如降低成本,可作為組織標本的補充。由于腫瘤異質性的存在,應儘可能穫取不同部位的腫瘤組織用于檢測。
목적다도경표본검측진전기비소세포폐암( aN-SCLC)환자표피생장인자수체( EGFR)돌변,급돌변상태여환자림상병이학특정、표본래원급혈청암배항원( CEA)적관계。방법수집aNSCLC환자조직、세포학급혈장표본,채용QIAGEN 공사신추출적 QIAamp Circulating Nucleic Acid Kit제취혈장순배유리DNA(cfDNA),사용갈형탐침확증조체돌변계통( sARMS)대제취DNA진행EGFR돌변검측。결과103례aNSCLC환자조직(혹세포)학표본검측EGFR돌변솔위48.5%(50/103),여흡연사、병리류형상관( P <0.05);여성별、년령、신체상태평분、분기、혈청 CEA 치무관;전이조조돌변솔(58.6%)교원발조조(35.6%)고,함유악성흉강적액(MPE)환자EGFR돌변솔(67.5%)교무MPE자(36.5%)고(P<0.05);29례혈장여조직(혹세포)학표본배대검측중,혈장양성솔위37.9%(11/29),민감성위43.7%(7/16),특이성위69.2%(9/13)。결론 aNSCLC환자EGFR돌변재비흡연、선암인군중고발,여성별、년령、혈CEA、분기、신체상태무관。전이조EGFR돌변솔교원발조고,병발MPE 환자EGFR 돌변솔교무MPE 자고。사용 QIAamp Circulating Nucleic Acid Kit 제취혈장cfDNA 검측 EGFR 돌변민감성상가,여강저성본,가작위조직표본적보충。유우종류이질성적존재,응진가능획취불동부위적종류조직용우검측。
Objective To detect epidermal growth factor receptor ( EGFR) mutations with multiple pathways speci-mens in advanced non-small cell lung cancer ( aNSCLC) patients,and study the relationship between EGFR muta-tion and specimen source,clinical features,pathological features,stage, the value of serum serum carcinoembryonic antigen (CEA). Methods Multiple pathways specimens like tumor tissue,cytology and plasma in aNSCLC pa-tients were collected. QIAGEN’s new QIAamp Circulating Nucleic Acid Kit was used to extract cfDNA in plasma, scorpion probe amplification refractory mutation system ( sARMS) was used for EGFR mutation test. Results The EGFR mutation rate of 103 cases in patients with NSCLC used tissue or cytology specimens was 48.5%(50/103). There was a significant correlation between EGFR mutation smoking history and histological type ( P<0.05 ) . No correlation with gender,age, physical status score, stage and the value of serum CEA. The mutation rate of metas-tases source group ( 58.6%) was higher than the primary tumor source group ( 35.6%) . Patients with malignant pleural effusions (67.5%) were higher than those without pleural effusion (36.5%) (P<0.05);verses tissue or cytology specimens,the EGFR mutation positive rate in 29 cases of paired plasma samples was 37.9%( 11/29 ) , sensitivity was 43.7%(7/16)and specificity was 69.2%(9/13). Conclusion aNSCLC patients with EGFR gene mutation were usually observed in non-smoking and adenocarcinoma patients. There is no significant difference with gender,serum carcinoembryonic antigen,clinical stage and physical status. The EGFR mutation rate is higher in pa-tients with malignant pleural effusion than patients without. Using QIAamp Circulating Nucleic Acid Kit for extrac-ting cfDNA in plasma to detect EGFR mutation,the sensitivity is acceptable. If cheap, plasma may be a comple-mentary approach for tumor tissue. Due to the existence of intratumoral EGFR mutational heterogeneity, obtaining different parts of the tumor tissue is essential.