安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
9期
1305-1309
,共5页
肝母细胞瘤%胸腺素beta-4%淋巴转移%儿童
肝母細胞瘤%胸腺素beta-4%淋巴轉移%兒童
간모세포류%흉선소beta-4%림파전이%인동
hepatoblastoma%thymosin beta 4%lymphnode metastasis%child
目的研究肝母细胞瘤( HB)患儿肝组织中胸腺素beta-4(Tβ4)表达水平及其临床病理意义。方法利用免疫组化法检测Tβ4、E-cadherin和β-catenin在19例儿童HB及其瘤旁对照肝组织中的表达水平，进一步探索Tβ4对HB转移的具体功能。结果 HB患儿肿瘤肝组织中Tβ4表达阳性率显著高于其远端瘤旁组织( P<0.01)。 Tβ4和细胞核β-catenin在淋巴结转移患者表达阳性率明显高于无淋巴结转移患者(P<0.01)，而E-cadherin表达量却明显降低(P<0.01)。体外划痕实验结果表明Tβ4敲除后的HepG2细胞迁移能力显著小于非敲除细胞( P<0.05)。结论 Tβ4在儿童HB淋巴转移中发挥重要功能，是治疗HB转移恶化的潜在分子靶点。
目的研究肝母細胞瘤( HB)患兒肝組織中胸腺素beta-4(Tβ4)錶達水平及其臨床病理意義。方法利用免疫組化法檢測Tβ4、E-cadherin和β-catenin在19例兒童HB及其瘤徬對照肝組織中的錶達水平，進一步探索Tβ4對HB轉移的具體功能。結果 HB患兒腫瘤肝組織中Tβ4錶達暘性率顯著高于其遠耑瘤徬組織( P<0.01)。 Tβ4和細胞覈β-catenin在淋巴結轉移患者錶達暘性率明顯高于無淋巴結轉移患者(P<0.01)，而E-cadherin錶達量卻明顯降低(P<0.01)。體外劃痕實驗結果錶明Tβ4敲除後的HepG2細胞遷移能力顯著小于非敲除細胞( P<0.05)。結論 Tβ4在兒童HB淋巴轉移中髮揮重要功能，是治療HB轉移噁化的潛在分子靶點。
목적연구간모세포류( HB)환인간조직중흉선소beta-4(Tβ4)표체수평급기림상병리의의。방법이용면역조화법검측Tβ4、E-cadherin화β-catenin재19례인동HB급기류방대조간조직중적표체수평，진일보탐색Tβ4대HB전이적구체공능。결과 HB환인종류간조직중Tβ4표체양성솔현저고우기원단류방조직( P<0.01)。 Tβ4화세포핵β-catenin재림파결전이환자표체양성솔명현고우무림파결전이환자(P<0.01)，이E-cadherin표체량각명현강저(P<0.01)。체외화흔실험결과표명Tβ4고제후적HepG2세포천이능력현저소우비고제세포( P<0.05)。결론 Tβ4재인동HB림파전이중발휘중요공능，시치료HB전이악화적잠재분자파점。
Objective To investigate the expression of thymosin beta4 ( Tβ4 ) in the liver tissues of children with hepatoblastoma ( HB) , and further study the function of Tβ4 in HB metastasis in vitro. Methods Immunohisto-chemistry (IHC) was used to determine expression of Tβ4, E-cadherin andβ-catenin in liver tissues from 19 chil-dren with HB,and further to analyze its function in metastasis of HB cells. Results The positive rate of Tβ4 ( P<0.01 ) in HB liver tissues was significantly higher than it in the adjacent tissues. The positive rates of Tβ4 and nu-clearβ-catenin in HB with lymphnode metastasis were significantly higher than in HB without lymphnode metastasis ( P <0.01 ) . While expression of E-cadherin in HB with lymphnode metastasis was lower than in HB without lymphnode metastasis ( P<0.01 ) . Scratch-wound assay showed that HepG2 cells with Tβ4 knockdown had signifi-cantly lower metastatic ability (P<0.05). Conclusion Tβ4 plays an important role in HB metastasis, suggesting it is a potential target for HB metastasis.
