CAJ | 학술논문

目的通过检测肿瘤转移相关基因2( MTA2)及p53在结直肠癌中的表达水平,探讨两者与结直肠癌各项临床病理因素之间的关系及临床意义。方法收集手术切除的120例结直肠癌标本,均取其原发病灶,并收集距肿瘤组织5 cm以上的非肿瘤性结直肠黏膜组织30例作为对照。用免疫组织化学法检测其MTA2、p53的表达。并根据不同的临床病理因素进行分组,分别比较两者的阳性表达与年龄、性别、病变部位、病理大体类型、浸润深度、有无淋巴结转移、有无远处转移及TNM分期的关系,分析其临床意义。结果免疫组化结果显示结直肠癌组织中 MTA2阳性表达率为59.16%(71/120),p53阳性表达率为61.67%(74/120),两者在正常结直肠黏膜组织表达率均为0,差异有统计学意义(P<0.01)。 MTA2阳性表达与肿瘤浸润深度、淋巴结转移、远处转移及TNM分期具有显著相关性( P<0.05),与其余各因素无显著相关性(P>0.05)。 p53阳性表达与肿瘤浸润深度、淋巴结转移有显著相关性(P<0.05),与其余各因素无显著相关性(P>0.05)。结论 MTA2可作为新的结直肠癌临床标志物及治疗靶点,其临床意义可能优于p53。
목적통과검측종류전이상관기인2( MTA2)급p53재결직장암중적표체수평,탐토량자여결직장암각항림상병리인소지간적관계급림상의의。방법수집수술절제적120례결직장암표본,균취기원발병조,병수집거종류조직5 cm이상적비종류성결직장점막조직30례작위대조。용면역조직화학법검측기MTA2、p53적표체。병근거불동적림상병리인소진행분조,분별비교량자적양성표체여년령、성별、병변부위、병리대체류형、침윤심도、유무림파결전이、유무원처전이급TNM분기적관계,분석기림상의의。결과면역조화결과현시결직장암조직중 MTA2양성표체솔위59.16%(71/120),p53양성표체솔위61.67%(74/120),량자재정상결직장점막조직표체솔균위0,차이유통계학의의(P<0.01)。 MTA2양성표체여종류침윤심도、림파결전이、원처전이급TNM분기구유현저상관성( P<0.05),여기여각인소무현저상관성(P>0.05)。 p53양성표체여종류침윤심도、림파결전이유현저상관성(P<0.05),여기여각인소무현저상관성(P>0.05)。결론 MTA2가작위신적결직장암림상표지물급치료파점,기림상의의가능우우p53。
Objective To determine the expression of metastasis-associated gene 2 ( MTA2 ) and p53 in colorectal cancer and non-cancerous mucosa, analyze their relationship with clinicopathological parameters, and discuss the clinical significance. Methods 120 colorectal neoplasm patients' cancer tissues and clinical information were col-lected from the first affiliated hospital of Anhui medical university. 30 patients of them were chosen to collect non-cancerous mucosa which was 5 cm away from the tumor. Immunohistochemistry staining was used to detect the ex-pression of MTA2 and p53. According to the clinicopathological parameters, the positive and negative expression of MTA2, p53 were counted each group, discussed the relationship between positive expression and clinicopathologi-cal parameters by SPSS 19.0 . Results The immunohistochemistry showed that the positive expression rate of MTA2 was 59.16%, and the positive expression rate of p53 was 61.67%. The expression of MTA2 and p53 in colorectal cancer were significantly higher than non-cancerous mucosa ( P<0.05 ) . The MTA2 expression was positively cor-related with the depth of invasion, lymphatic metastasis, distant metastasis and TNM stages (P<0.05). The p53 protein expression was positively correlated with depth of invasion and lymphatic metastasis ( P<0.05 ) . Conclu-sion MTA2 could be used as a new clinical biomarker and therapeutic target for colorectal cancer probably, which might be more effective than p53 .