安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
9期
1295-1297
,共3页
CD146 mRNA%肾透明细胞癌%肿瘤组织
CD146 mRNA%腎透明細胞癌%腫瘤組織
CD146 mRNA%신투명세포암%종류조직
CD146 mRNA%renal clear cell carcinoma%tumor tissue
目的探讨肾透明细胞癌( CCRCC)患者肿瘤组织中CD146 mRNA的表达及意义。方法用ELISA法定量检测CD146蛋白含量及Real-Time PCR技术检测102例CCRCC患者肿瘤组织中CD146 mRNA的表达,并以51例肾脏良性病变患者手术正常肾组织作为对照。结果发现存在转移的CCRCC患者, CD146蛋白浓度与对照组比较差异有统计学意义(F =52.1,P <0.01),CD146 mRNA 的平均表达值(0.0438±0.0024)明显高于原位CCRCC患者(0.0382±0.0011,P=0.018)和对照组患者(0.0344±0.0010,P=0.001)。结论 CD146 mRNA表达上调与CCRCC的病理分级及淋巴结转移相关,有望成为评判CCRCC恶性程度、转移潜能及预后的一个新指标,为临床的干预性治疗提供可靠±据。
目的探討腎透明細胞癌( CCRCC)患者腫瘤組織中CD146 mRNA的錶達及意義。方法用ELISA法定量檢測CD146蛋白含量及Real-Time PCR技術檢測102例CCRCC患者腫瘤組織中CD146 mRNA的錶達,併以51例腎髒良性病變患者手術正常腎組織作為對照。結果髮現存在轉移的CCRCC患者, CD146蛋白濃度與對照組比較差異有統計學意義(F =52.1,P <0.01),CD146 mRNA 的平均錶達值(0.0438±0.0024)明顯高于原位CCRCC患者(0.0382±0.0011,P=0.018)和對照組患者(0.0344±0.0010,P=0.001)。結論 CD146 mRNA錶達上調與CCRCC的病理分級及淋巴結轉移相關,有望成為評判CCRCC噁性程度、轉移潛能及預後的一箇新指標,為臨床的榦預性治療提供可靠±據。
목적탐토신투명세포암( CCRCC)환자종류조직중CD146 mRNA적표체급의의。방법용ELISA법정량검측CD146단백함량급Real-Time PCR기술검측102례CCRCC환자종류조직중CD146 mRNA적표체,병이51례신장량성병변환자수술정상신조직작위대조。결과발현존재전이적CCRCC환자, CD146단백농도여대조조비교차이유통계학의의(F =52.1,P <0.01),CD146 mRNA 적평균표체치(0.0438±0.0024)명현고우원위CCRCC환자(0.0382±0.0011,P=0.018)화대조조환자(0.0344±0.0010,P=0.001)。결론 CD146 mRNA표체상조여CCRCC적병리분급급림파결전이상관,유망성위평판CCRCC악성정도、전이잠능급예후적일개신지표,위림상적간예성치료제공가고±거。
Objective To explore renal clear cell carcinoma ( CCRCC) expression and significance of CD146 mR-NA in tumor tissue of patients with. Methods ELISA method for quantitative detection was used for CD146 protein and real-time PCR technique for the detection of CCRCC in 102 ( CCRCC) expression in tumor tissue of patients with CD146 mRNA, and 51 cases of renal patients with non tumor tissues as control. Results Found metastasis in patients with CCRCC, the CD146 protein concentration was statistically significant compared with the control group (F=52.1, P<0.01),the average expression of CD146 mRNA value (0.043 8±0.002 4) was significantly high-er than that of in situ CCRCC patients (0.038 2±0.001 1, P= 0.018) and control group (0.034 4±0.001 0, P=0.001 ) . Conclusion Pathological grading and lymph node up regulation of CD146 mRNA expression in renal cell carcinoma and metastasis, is expected to become a new index, evaluation of malignant degree of CCRCC me-tastasis and prognosis, and provide a reliable basis for the intervention of clinical treatment.
