CAJ | 학술논문

建立高效液相色谱法同时测定长座虫草中13种核苷和碱基类物质,色谱条件为：TSKgel ODS-100V色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.01 mol/L KH2PO4水溶液系统为流动相,梯度洗脱,流速1 ml/min,检测波长260 nm,柱温20℃。13种成分在50 min内均达到基线分离,线性关系良好(r≥0.9990,n=8)；平均回收率在98.4%~107.5%(RSD<2.0%,n=5)。结果表明长座虫草子实体中胞嘧啶、次黄嘌呤含量相对较高；菌丝体中尿嘧啶、鸟苷、腺苷含量较高,尿苷在菌丝体和子实体中含量均较高。
건립고효액상색보법동시측정장좌충초중13충핵감화감기류물질,색보조건위：TSKgel ODS-100V색보주(250 mm×4.6 mm,5μm),이갑순-0.01 mol/L KH2PO4수용액계통위류동상,제도세탈,류속1 ml/min,검측파장260 nm,주온20℃。13충성분재50 min내균체도기선분리,선성관계량호(r≥0.9990,n=8)；평균회수솔재98.4%~107.5%(RSD<2.0%,n=5)。결과표명장좌충초자실체중포밀정、차황표령함량상대교고；균사체중뇨밀정、조감、선감함량교고,뇨감재균사체화자실체중함량균교고。
An HPLC method was established for simultaneous determination of 13 nucleosides and nucleobases in Ophiocordyceps longissima. The separation was performed on a TSK gel ODS-100V column (250 mmí4.6 mm,5μm) using gradient elution with an aqueous potassium dihydrogen phosphate-methanol solution at 260 nm. The flow rate was 1 . 0 ml/min and column temperature was 20℃. Thirteen nucleosides and nucleobases were analyzed with-in 50 min. The calibration curves showed good linear relationship (r≥0. 999 0, n=8) and the method exhibited excellent accuracy with a recovery of 98. 4 to 107. 5% (RSD<2. 0%,n=5). Higher content of cytosine and hy-poxanthine were detected in the fruiting body. The contents of adenosine, uracil, guanosine were relatively rich in mycelium of Ophiocordyceps longissima. Uridine was abundant both in fruiting body and mycelium of Ophiocordyceps longissima.