CAJ | 학술논문

目的检测小鼠经三氯乙烯( TCE )致敏后Tc1、Tc2细胞的变化,探讨Tc1/Tc2细胞失衡在三氯乙烯药疹样皮炎( DMLT)中的可能作用。方法 BALB/c雌性小鼠随机分为空白对照、溶剂对照和TCE处理组,在末次激发后24、48、72 h和7d根据皮肤肿胀程度和病理表现分为致敏组和未致敏组。无菌取出脾脏,用流式细胞术检测Tc1、Tc2细胞数量,实时荧光定量PCR检测T-bet、GATA-3 mRNA转录水平。结果与未致敏组相比,致敏组Tc1细胞数量在24、48、72 h和7 d显著上升(P<0.01),72 h水平最高；致敏组Tc2细胞数量在24、48、72 h显著上升(P<0.01),72 h水平达到峰值,7 d较未致敏组差异无统计学意义( P >0.05)；致敏组Tc1/ Tc2比例于48、72 h 和7 d 较未致敏组显著上升(P <0.01)。致敏组T-bet、GATA-3 mRNA 转录水平在48、72 h 和7 d 较相应时点未致敏组显著上升(P <0.05,P <0.01),72 h 达到峰值。结论Tc1/ Tc2细胞失衡可能参与了TCE 致敏小鼠的免疫损伤过程并发挥重要的作用。
목적검측소서경삼록을희( TCE )치민후Tc1、Tc2세포적변화,탐토Tc1/Tc2세포실형재삼록을희약진양피염( DMLT)중적가능작용。방법 BALB/c자성소서수궤분위공백대조、용제대조화TCE처리조,재말차격발후24、48、72 h화7d근거피부종창정도화병리표현분위치민조화미치민조。무균취출비장,용류식세포술검측Tc1、Tc2세포수량,실시형광정량PCR검측T-bet、GATA-3 mRNA전록수평。결과여미치민조상비,치민조Tc1세포수량재24、48、72 h화7 d현저상승(P<0.01),72 h수평최고；치민조Tc2세포수량재24、48、72 h현저상승(P<0.01),72 h수평체도봉치,7 d교미치민조차이무통계학의의( P >0.05)；치민조Tc1/ Tc2비례우48、72 h 화7 d 교미치민조현저상승(P <0.01)。치민조T-bet、GATA-3 mRNA 전록수평재48、72 h 화7 d 교상응시점미치민조현저상승(P <0.05,P <0.01),72 h 체도봉치。결론Tc1/ Tc2세포실형가능삼여료TCE 치민소서적면역손상과정병발휘중요적작용。
Objective To detect the alterations of Tc1 and Tc2 cells in trichloroethylene ( TCE ) sensitized mice and to shed light on the possible role of Tc1/Tc2 imbalance on occupational medicamentosa-like dermatitis induced by trichloroethylene ( DMLT) . Methods Female BALB/c mice, randomly separated into blank control, solvent control and TCE group, were sacrificed on 24, 48, 72 h and 7 d after last challenge. Sensitized or non-sensitized mice were identified by cutaneous swelling and histological appearance before sacrifice. Spleens were taken to pre-pare splenocyte suspensions quantifying Tc1 and Tc2 by flow cytometry. Tc lineage-specific transcription factors, T-bet and GATA-3, were evaluated by real-time fluorescence quantitative PCR. Results Compared with non-sensi-tized group, the percentages of Tc1 cells in sensitized mice were significantly higher at four consecutive timepoints (P<0.01), which peaked at 72 h. Tc2 cell levels in sensitized mice were markedly elevated at 24 and 48 h with reaching the summit at 72 h ( P<0.01 ) , whereas the level at 7 d showed no significant difference with non-sensi-tized mice ( P>0.05 ) . Tc1/Tc2 ratios in sensitized group were significantly higher at 48 , 72 h and 7 d compared with non-sensitized group. The levels of T-bet and GATA-3 mRNA in sensitized mice were remarkably increased than corresponding non-sensitized mice at 48 , 72 h and 7 d ( P<0.05 , P<0.01 ) . Conclusion The imbalance of Tc1/Tc2 cells may play a key role in the immunological damage in TCE sensitized mice.