CAJ | 학술논문

目的探讨普萘洛尔对人增生期血管瘤裸鼠种植瘤生长的影响。方法将手术切除的1例儿童增生期毛细血管瘤瘤体组织分切成组织块，分别植入16只幼小裸鼠( BALB/c nu/nu)的皮下，每只4处。建立人增生期血管瘤裸鼠模型，于第45天随机分成两组：普萘洛尔组的每只裸鼠用0.01%普萘洛尔溶液0.4 ml灌胃，每天1次，连续1周；给予同样体积的蒸馏水每天1次，连续1周灌胃作为对照组。密切观察裸鼠及移植瘤体的生长情况并做相应记录，通过免疫组织化学方法以及Western blot方法分别检测血管内皮生长因子( VEGF)及胰岛素样生长因子-2( IGF-2)的表达情况。结果普萘洛尔组裸鼠的肿瘤体积在干预期内明显下降，而对照组瘤体体积增加。使用普萘洛尔后，移植血管瘤VEGF、IGF-2蛋白的表达明显减弱。结论普萘洛尔可以有效地抑制人血管瘤移植瘤生长速度，抑制VEGF和IGF-2的分泌，从而有效抑制肿瘤血管生成，加速肿瘤转化为消退期。
목적탐토보내락이대인증생기혈관류라서충식류생장적영향。방법장수술절제적1례인동증생기모세혈관류류체조직분절성조직괴，분별식입16지유소라서( BALB/c nu/nu)적피하，매지4처。건립인증생기혈관류라서모형，우제45천수궤분성량조：보내락이조적매지라서용0.01%보내락이용액0.4 ml관위，매천1차，련속1주；급여동양체적적증류수매천1차，련속1주관위작위대조조。밀절관찰라서급이식류체적생장정황병주상응기록，통과면역조직화학방법이급Western blot방법분별검측혈관내피생장인자( VEGF)급이도소양생장인자-2( IGF-2)적표체정황。결과보내락이조라서적종류체적재간예기내명현하강，이대조조류체체적증가。사용보내락이후，이식혈관류VEGF、IGF-2단백적표체명현감약。결론보내락이가이유효지억제인혈관류이식류생장속도，억제VEGF화IGF-2적분비，종이유효억제종류혈관생성，가속종류전화위소퇴기。
Objective To discuss the effect of propranolol on the proliferation of human hemangioma on growth of planting tumor in nude mice. Methods Surgery on 1 case of proliferative phase for children strawberry hemangio-ma tissue fragments made from, respectively, 16 pups in nude mice implanted ( BALB/c nu/nu) under the skin on each of 4 . Establishment of nude mouse model of human hyperplastic vascular tumor was randomly divided into two groups on the 45th day:In general group of propranolol, propranolol was given with 0. 01% solution 0. 4 ml after intragastric infusion once a day for a week. In mice of control group, intragastric administration of the same volume of distilled water was given once a day for a week. The growth conditions of the nude mice and transplanted tumors were observed, using the method of immunohistochemistry and Western blot to detect the expression of vascular en-dothelial growth factor (VEGF) and insulin-like growth factor-2 (IGF-2). Results Experimental group in nude mice tumor volume in the intervening period was declined significantly, while the tumor size in the control group was increaseing. After intragastric administration of propranolol, the expression of VEGF and IGF-2 protein in graft tumor was decreased significantly. Conclusion Propranolol can inhibits the growth of human hemangioma growing speed in vivo and inhibits the secretion of VEGF and IGF-2. With decreased VEGF and IGF-2 secretion,the tumor angiogenesis is inhibited and the fade transition is accelerated.