CAJ | 학술논문

目的探讨环氧合酶2( COX2)抑制剂NS-398对卵巢癌细胞恶性生物学特性的影响。方法以卵巢癌细胞株SKOV3为研究对象,根据 NS-398的处理分为对照组、实验组。利用MTS法和免疫组化法检测Ki-67蛋白表达,从而监测NS-398对SKOV3细胞增殖的影响；采用划痕实验和侵袭实验分别观察细胞迁移和侵袭能力的变化,采用RT-PCR法检测基质金属蛋白酶( MMP ) mRNA的表达情况；采用实时荧光定量PCR及Western blot检测上皮间质转化( EMT)相关分子表达情况。结果① MTS增殖实验表明NS-398对SKOV3细胞增殖作用无影响,免疫组化结果与之一致；②划痕实验和侵袭实验分别显示,NS-398显著下调SKOV3细胞的迁移和侵袭能力,差异有统计学意义(P<0.05)。 RT-PCR检测结果表明NS-398抑制MMP的mRNA表达；③ Western blot结果表明NS-398上调 SKOV3细胞 E-cadherin的表达,下调Vimentin、Slug、Snail的表达。结论 COX2抑制剂通过阻止MMP 的表达和EMT的发生,从而下调SKOV3细胞的迁移、侵袭能力。
목적탐토배양합매2( COX2)억제제NS-398대란소암세포악성생물학특성적영향。방법이란소암세포주SKOV3위연구대상,근거 NS-398적처리분위대조조、실험조。이용MTS법화면역조화법검측Ki-67단백표체,종이감측NS-398대SKOV3세포증식적영향；채용화흔실험화침습실험분별관찰세포천이화침습능력적변화,채용RT-PCR법검측기질금속단백매( MMP ) mRNA적표체정황；채용실시형광정량PCR급Western blot검측상피간질전화( EMT)상관분자표체정황。결과① MTS증식실험표명NS-398대SKOV3세포증식작용무영향,면역조화결과여지일치；②화흔실험화침습실험분별현시,NS-398현저하조SKOV3세포적천이화침습능력,차이유통계학의의(P<0.05)。 RT-PCR검측결과표명NS-398억제MMP적mRNA표체；③ Western blot결과표명NS-398상조 SKOV3세포 E-cadherin적표체,하조Vimentin、Slug、Snail적표체。결론 COX2억제제통과조지MMP 적표체화EMT적발생,종이하조SKOV3세포적천이、침습능력。
Objective To investigate effects of cyclooxygenase-2 ( COX2 ) inhibitor NS-398 on the biological char-acteristics of malignant ovarian cancer. Methods This study had ovarian cancer cell line SKOV3 as the research object. According to NS-398 treatment, this study was divided into control group, the experimental group. The use of MTS method and immunohistochemical expression of Ki-67 predicted the effect of NS-398 on proliferation and growth of SKOV3 cell, and scratch experiments and invasion assay to respectively detect the changes of migration and invasiveness capability and analysis of RT-PCR in matrix metalloproteinase ( MMP) mRNA, and Western blot to detect the expression of epithelial-mesenchymal transition( EMT) related molecule. Results ① MTS prolifera-tion assay suggested that NS-398 had no influence on proliferation of SKOV3 cell ( P>0.05 ) , and consistent with the results of immunohistochemistry. ②Scratch experiments and invasion assay respectively showed NS-398 signifi-cantly down-regulate invasiveness and migration of SKOV3 cells, the differences were statistically significant ( P<0.05) . RT-PCR test results showed that NS-398 inhibited the mRNA expression of MMP. ③ In Western blot, it was found that NS-398 could increase E-cadherin expression and reduce Vimentin, slug, snail expression in ovarian cancer cells. Conclusion NS-398 inhibits migration and invasion of SKOV3 cell by down-regulating expression of MMP and preventing the occurrence of EMT.