检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
18期
2510-2512
,共3页
蒋红%雷佳红%何城%郭晓兰%杨明辉%蔡燕
蔣紅%雷佳紅%何城%郭曉蘭%楊明輝%蔡燕
장홍%뢰가홍%하성%곽효란%양명휘%채연
苦参碱%人成纤维样滑膜细胞%类风湿关节炎
苦參堿%人成纖維樣滑膜細胞%類風濕關節炎
고삼감%인성섬유양활막세포%류풍습관절염
matrine%human fibroblast-like synovial cells%rheumatoid arthritis
目的:探讨苦参碱对类风湿关节炎的潜在治疗作用。方法原代培养人关节成纤维样滑膜细胞,传至第3~5代时,以0.4、0.8、1.2、1.6、2.0mg/mL等不同浓度的苦参碱处理细胞,分别于作用后24、48h用倒置显微镜观察细胞形态。采用四甲基偶氮唑蓝(MTT )法检测不同浓度苦参碱处理的细胞生长情况,计算抑制率。结果不同浓度的苦参碱均可抑制人成纤维样滑膜细胞生长,MTT 检测结果显示细胞抑制率为2.52%~25.74%,随苦参碱药物浓度增加和处理时间延长,抑制作用增强。结论苦参碱可抑制人成纤维样滑膜细胞生长,可作为治疗类风湿关节炎的潜在药物。
目的:探討苦參堿對類風濕關節炎的潛在治療作用。方法原代培養人關節成纖維樣滑膜細胞,傳至第3~5代時,以0.4、0.8、1.2、1.6、2.0mg/mL等不同濃度的苦參堿處理細胞,分彆于作用後24、48h用倒置顯微鏡觀察細胞形態。採用四甲基偶氮唑藍(MTT )法檢測不同濃度苦參堿處理的細胞生長情況,計算抑製率。結果不同濃度的苦參堿均可抑製人成纖維樣滑膜細胞生長,MTT 檢測結果顯示細胞抑製率為2.52%~25.74%,隨苦參堿藥物濃度增加和處理時間延長,抑製作用增彊。結論苦參堿可抑製人成纖維樣滑膜細胞生長,可作為治療類風濕關節炎的潛在藥物。
목적:탐토고삼감대류풍습관절염적잠재치료작용。방법원대배양인관절성섬유양활막세포,전지제3~5대시,이0.4、0.8、1.2、1.6、2.0mg/mL등불동농도적고삼감처리세포,분별우작용후24、48h용도치현미경관찰세포형태。채용사갑기우담서람(MTT )법검측불동농도고삼감처리적세포생장정황,계산억제솔。결과불동농도적고삼감균가억제인성섬유양활막세포생장,MTT 검측결과현시세포억제솔위2.52%~25.74%,수고삼감약물농도증가화처리시간연장,억제작용증강。결론고삼감가억제인성섬유양활막세포생장,가작위치료류풍습관절염적잠재약물。
Objective To investigate the potential therapeutical effect of matrine on patients with rheumatoid arthritis(RA) .Methods The human fibroblast-like synovial cells were collected from patients with RA and cultured to 3th to 5th generation ,then treated with different concentrations of matrine (0 .4 ,0 .8 ,1 .2 ,1 .6 ,2 .0 mg/mL) .Mi-croscope was used to observe the shape of cells after treating 24 hours or 48 hours .The methyl thiazolyl tetrazolium (MTT)method was used to detect the inhibitor rates of cells in different concentrations of matrine .Results Matrine could inhabit the growth of human fibroblast-like synovial .The result of MTT method showed the inhibition rate was 2 .52% -25 .74% under different concentrations of matrine ,as the incubated concentration and incubated time in-creased ,the inhibition rate increased too .Conclusion Matrine could inhibit the growth of human fibroblast-like syno-vial cells ,which could be used as a potential drug for patients with RA .