CAJ | 학술논문

目的：研究 t4606基因在伤寒沙门菌适应高渗应激环境时的生物学作用，为临床治疗和预防伤寒沙门菌感染提供可能的靶向基因。方法采用原核生物基因同源重组技术制备伤寒沙门菌 t4606基因缺陷株及t4606基因缺陷回补株；以生长时间为横坐标，培养液600 nm处得吸光度值为纵坐标，绘制生长曲线，比较 t4606基因缺陷株、回补株及野生株在高渗应激（300 mmol／L NaCl）环境下的生长能力；统计学分析不同菌株间的吸光度值差异。结果成功制备伤寒沙门菌 t4606基因缺陷株和相应的回补株；生长曲线分析显示在高渗应激环境下， t4606基因缺陷株的生存能力明显弱于野生株。结论 t4606基因在伤寒沙门菌适应高渗应激环境方面发挥着重要的作用，t4606基因有可能作为临床治疗和预防伤寒沙门菌感染的靶向基因。
목적：연구 t4606기인재상한사문균괄응고삼응격배경시적생물학작용，위림상치료화예방상한사문균감염제공가능적파향기인。방법채용원핵생물기인동원중조기술제비상한사문균 t4606기인결함주급t4606기인결함회보주；이생장시간위횡좌표，배양액600 nm처득흡광도치위종좌표，회제생장곡선，비교 t4606기인결함주、회보주급야생주재고삼응격（300 mmol／L NaCl）배경하적생장능력；통계학분석불동균주간적흡광도치차이。결과성공제비상한사문균 t4606기인결함주화상응적회보주；생장곡선분석현시재고삼응격배경하， t4606기인결함주적생존능력명현약우야생주。결론 t4606기인재상한사문균괄응고삼응격배경방면발휘착중요적작용，t4606기인유가능작위림상치료화예방상한사문균감염적파향기인。
Objective To investigate the biological effect of t4606 gene in Salmonella typhi (S .typhi) under hyperosmotic stress and provide target genes for therapy and prevention of infection disease caused by S .typhi . Methods t4606 deleted strains (Δt4606 strains) of S .typhi was prepared by homologious recombination .Supplemen-tal strains Δt4606 (pBADt4606) was also prepared with recombinant plasmid pBAD t4606 in the Δt4606 strains . Growth curves ware drawn as the X-axis with time and the Y-axis with the absorbance value at 600 nm .The survival ability of Δt4606 strains ,Δt4606 (pBADt4606) strains and wild-type strains under high osmolarity stress (300 mmol/L NaCl) were compared .The differences were statistically analyzed .Results The Δt4606 strains and Δt4606 (pBAD t4606) strains were successfully generated .Compared with wild-type strains ,the survival ability of Δt4606 strains was significantly compromised under high osmolarity stress .Conclusion It could be confirmed that t4606 gene might be important for S .typhi to overcome the high osmolarity ,which indicate that t4606 gene could be a target gene for therapy and prevention of infection diseases caused by S .typhi .