中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2014年
27期
3217-3219,3220
,共4页
高炳玉%刘玉%夏立平%郑武平%苏洁之%陈峙霖
高炳玉%劉玉%夏立平%鄭武平%囌潔之%陳峙霖
고병옥%류옥%하립평%정무평%소길지%진치림
肺肿瘤%胸苷酸合酶%基因表达%培美曲塞%化学疗法,辅助
肺腫瘤%胸苷痠閤酶%基因錶達%培美麯塞%化學療法,輔助
폐종류%흉감산합매%기인표체%배미곡새%화학요법,보조
Lung neoplasms%Thymidylate synthase%Gene expression%Pemetrexed%Chemotherapy,adjuvant
目的：探讨海南黎族肺腺癌患者癌组织中胸苷酸合酶（ TS） mRNA表达水平对培美曲塞辅助化疗预后的影响。方法选取2008年6月-2009年4月在我院手术治疗的晚期肺腺癌患者52例，采用培美曲塞为主的方案进行辅助化疗。采用反转录聚合酶链反应（ RT-PCR）和相对定量的方法分别检测患者癌组织及癌旁组织中TS mRNA的表达水平，分析TSmRNA表达水平对患者化疗预后的影响。结果52例肺腺癌患者中，35例（67.3%）癌组织TS mRNA表达水平低于其癌旁组织。52例肺腺癌患者癌组织中TS基因 mRNA表达水平相差67倍，以临界值（ cut-off均值）为界，TS mRNA高表达患者29例（55.8%），低表达患者23例（44.2%）。癌组织中TS mRNA低表达组患者的无复发生存率（61.0%）和总生存率（56.5%）均高于高表达组（39.0%和43.5%）（P<0.05）。结果癌组织中TS mRNA低表达的患者更能从培美曲塞为主的辅助化疗方案中受益；检测癌组织中TS mRNA表达水平有助于预测患者对化疗药物的敏感性。
目的：探討海南黎族肺腺癌患者癌組織中胸苷痠閤酶（ TS） mRNA錶達水平對培美麯塞輔助化療預後的影響。方法選取2008年6月-2009年4月在我院手術治療的晚期肺腺癌患者52例，採用培美麯塞為主的方案進行輔助化療。採用反轉錄聚閤酶鏈反應（ RT-PCR）和相對定量的方法分彆檢測患者癌組織及癌徬組織中TS mRNA的錶達水平，分析TSmRNA錶達水平對患者化療預後的影響。結果52例肺腺癌患者中，35例（67.3%）癌組織TS mRNA錶達水平低于其癌徬組織。52例肺腺癌患者癌組織中TS基因 mRNA錶達水平相差67倍，以臨界值（ cut-off均值）為界，TS mRNA高錶達患者29例（55.8%），低錶達患者23例（44.2%）。癌組織中TS mRNA低錶達組患者的無複髮生存率（61.0%）和總生存率（56.5%）均高于高錶達組（39.0%和43.5%）（P<0.05）。結果癌組織中TS mRNA低錶達的患者更能從培美麯塞為主的輔助化療方案中受益；檢測癌組織中TS mRNA錶達水平有助于預測患者對化療藥物的敏感性。
목적：탐토해남려족폐선암환자암조직중흉감산합매（ TS） mRNA표체수평대배미곡새보조화료예후적영향。방법선취2008년6월-2009년4월재아원수술치료적만기폐선암환자52례，채용배미곡새위주적방안진행보조화료。채용반전록취합매련반응（ RT-PCR）화상대정량적방법분별검측환자암조직급암방조직중TS mRNA적표체수평，분석TSmRNA표체수평대환자화료예후적영향。결과52례폐선암환자중，35례（67.3%）암조직TS mRNA표체수평저우기암방조직。52례폐선암환자암조직중TS기인 mRNA표체수평상차67배，이림계치（ cut-off균치）위계，TS mRNA고표체환자29례（55.8%），저표체환자23례（44.2%）。암조직중TS mRNA저표체조환자적무복발생존솔（61.0%）화총생존솔（56.5%）균고우고표체조（39.0%화43.5%）（P<0.05）。결과암조직중TS mRNA저표체적환자경능종배미곡새위주적보조화료방안중수익；검측암조직중TS mRNA표체수평유조우예측환자대화료약물적민감성。
Objective Toinvestigatetheprognosticimpactofthymidylatesynthase(TS)mRNAexpressionlevelin HainanLinationalitylungadenocarcinomapatientstreatedwithpemetrexed.Methods Atotalof52patientswithadvancedlung adenocarcinoma who had surgery in our hospital from June 2008 to April 2009,were selected as study subjects. All patients re-ceived adjuvant chemotherapy,pemetrexed was the main chemotherapeutic drug. RT -PCR and relative quantification method were used to detect the expression levels of cancer tissues and paracancerous tissues. The prognostic impact of TS mRNA expres-sionlevelinpatientswhoreceivedchemotherapywasanalyzed.Results Of52patients,TSmRNAexpressionlevelsofcancer tissues were significantly lower than those of paracancerous tissues in 35 cases(67. 3%). Of 52 patients,the highest TS mRNA expression level was 67 times as much as the lowest TS mRNA expression level of cancer tissues. According to median value of cut-off value,there were 29 patients(55. 8%)with high expression of TS gene mRNA in carcinoma tissue,and there were 23 pa-tients(44. 2%)with low expression. The recurrent-free survival rate(61. 0%)and overall survival rate(56. 5%)of patients in low TS mRNA expression level group were significantly higher than those(39. 0% and 43. 5%) of patients in high TS mRNA expressionlevelgroup,respectively(P<0.05).℅onclusion ThepatientswithlowexpressionlevelofTSmRNAincarcinoma tissue can benefit from adjuvant chemotherapy with pemetrexed. Detection of TS mRNA expression levels in cancer tissues can pre-dict the sensitivity to chemotherapeutic drugs.
