CAJ | 학술논문

目的：探讨多巴胺激动D1受体在重复给予亚惊厥剂量戊四氮（ PTZ）所诱导的大鼠全身性癫痫持续状态（ SE）模型中所起的作用。方法二级健康雄性SD大鼠40只，按照随机数字表法分为PTZ组、LSP组、LSSP组和对照组，每组10只。PTZ组大鼠接受重复低剂量的PTZ诱发SE；LSP组大鼠首先接受舒必利、左旋多巴腹腔注射，再接受重复低剂量的PTZ诱发至SE；LSSP组大鼠首先接受舒必利+SCH23390、左旋多巴腹腔注射，再接受重复低剂量的PTZ诱发至SE；对照组大鼠接受与PTZ组相同次数相同体积的0.9%氯化钠溶液注射，2 h处死大鼠。比较各组诱导不同程度癫痫发作所用PTZ累积剂量，免疫组织化学染色法检测Fos在皮质、海马及纹状体的表达数量。结果对照组大鼠无癫痫发作。PTZ组、LSP组、LSSP组诱导不同级别癫痫发作所用 PTZ累积剂量比较，差异均有统计学意义（P<0.05）；其中LSP组较PTZ组和LSSP组的用量降低（P<0.05）；PTZ组与LSSP组比较，差异均无统计学意义（P>0.05）。3组组内比较，诱导不同级别癫痫发作所用PTZ累积剂量差异均有统计学意义（P<0.05）。PTZ组、LSP组、LSSP组Fos在皮质、海马及纹状体表达数量比较，差异均无统计学意义（ P>0.05）。结论多巴胺激动D1受体对PTZ诱导的SE有促惊厥作用，并贯穿于癫痫发生、发展的全过程。
목적：탐토다파알격동D1수체재중복급여아량궐제량무사담（ PTZ）소유도적대서전신성전간지속상태（ SE）모형중소기적작용。방법이급건강웅성SD대서40지，안조수궤수자표법분위PTZ조、LSP조、LSSP조화대조조，매조10지。PTZ조대서접수중복저제량적PTZ유발SE；LSP조대서수선접수서필리、좌선다파복강주사，재접수중복저제량적PTZ유발지SE；LSSP조대서수선접수서필리+SCH23390、좌선다파복강주사，재접수중복저제량적PTZ유발지SE；대조조대서접수여PTZ조상동차수상동체적적0.9%록화납용액주사，2 h처사대서。비교각조유도불동정도전간발작소용PTZ루적제량，면역조직화학염색법검측Fos재피질、해마급문상체적표체수량。결과대조조대서무전간발작。PTZ조、LSP조、LSSP조유도불동급별전간발작소용 PTZ루적제량비교，차이균유통계학의의（P<0.05）；기중LSP조교PTZ조화LSSP조적용량강저（P<0.05）；PTZ조여LSSP조비교，차이균무통계학의의（P>0.05）。3조조내비교，유도불동급별전간발작소용PTZ루적제량차이균유통계학의의（P<0.05）。PTZ조、LSP조、LSSP조Fos재피질、해마급문상체표체수량비교，차이균무통계학의의（ P>0.05）。결론다파알격동D1수체대PTZ유도적SE유촉량궐작용，병관천우전간발생、발전적전과정。
Objective ToinvestigatethepromotingconvulsionsroleofdopamineexciteD1receptorinratswithpentet-razol-inducedgeneralizedstatusepilepticus(SE).Methods Atotalof40gradetwohealthymaleSDratsweredividedinto pentetrazol(PTZ)group,LSP group,LSSP group and control group by random number table method,10 in each group. The SE of rats in PTZ group was induced by repeated low-dose PTZ. Sulpiride and levodopa were injected into the abdominal cavity of rats in LSP group,then the SE of rats in LSP group was induced by repeated low-dose PTZ. Sulpiride,SCH23390 and levodopa were injected into the abdominal cavity of rats in LSSP group,then the SE of rats in LSSP group was induced by repeated low-dose PTZ. Rats in control group were injected with 0. 9% sodium chloride solution,the injection frequency and volume in control group were equal to those in PTZ group,all rats were put to death after 2 hours. The accumulated doses of PTZ were compared a-mong different groups,the Fos expression in cortex,hippocampus,and corpus striatum was detected by immunohistochemis-try.Results ThereweresignificantdifferencesintheaccumulateddosesofPTZamongPTZgroup,LSPgroupandLSSPgroup (P<0. 05). The PTZ dose in LSP group was significantly lower than that in PTZ group and in LSSP group(P<0. 05). There was no significant difference in the PTZ dose between PTZ group and LSSP group(P>0. 05). There were significant differences in epileptic attack classification among PTZ group,LSP group and LSSP group(P<0. 05). There was no significant difference inquantityofFosexpressionincortex,hippocampus,andcorpusstriatum(P>0.05).℅onclusion ThedopamineexciteD1 receptor could promote convulsions in rats with PTZ-induced SE,it runs through all the process of occurrence and development of epilepsy.