CAJ | 학술논문

试验以新生牛睾丸组织、牛胎儿皮肤组织为材料，进行支持细胞、成纤维细胞的分离、纯化、鉴定及pEGFP-N3载体转染。结果表明，完善犊牛支持细胞、胎牛皮肤成纤维细胞体外培养体系，细胞纯度可达95%。支持细胞在相同密度下较成纤维细胞能更快完成细胞生长指数期，生长迅速。转染pEGFP-N3载体后，初期EGFP均匀充满胞浆，后期EGFP向细胞核聚集，荧光强度明显高于细胞质中。转染后24、48、72 h，成纤维细胞转染效率高于支持细胞，转染pEGFP-N3的成纤维细胞和支持细胞在转染48 h后达到最大转染效率。转染后成纤维细胞的存活时间明显高于支持细胞，这与支持细胞生物学性质有关。
시험이신생우고환조직、우태인피부조직위재료，진행지지세포、성섬유세포적분리、순화、감정급pEGFP-N3재체전염。결과표명，완선독우지지세포、태우피부성섬유세포체외배양체계，세포순도가체95%。지지세포재상동밀도하교성섬유세포능경쾌완성세포생장지수기，생장신속。전염pEGFP-N3재체후，초기EGFP균균충만포장，후기EGFP향세포핵취집，형광강도명현고우세포질중。전염후24、48、72 h，성섬유세포전염효솔고우지지세포，전염pEGFP-N3적성섬유세포화지지세포재전염48 h후체도최대전염효솔。전염후성섬유세포적존활시간명현고우지지세포，저여지지세포생물학성질유관。
The newborn calf testicles and embryo skin were used in this experiment, the sertoli cells and fibroblast cells were transfected with the plasmid pEGFP-N3 by Liposome-mediated. The results showed that the purity of the cells was up to 95%. The early EGFP was evenly fil ed in cytosol after transfection. Later, EGFP was gathered in the nucleus and the fluorescence intensity in the nucleus became more higher in the cytoplasm. The results showed that transfection efficiency of fibroblast cellwas higher than sertoli cells. The transfection efficiency of fibroblast cells and sertoli cells was significantly different after transfection at 24, 48 and 72 h. Both cells got the highest transfection efficiency at 48 h. After transfecting, the life of fibroblast cells was obviously longer than sertoli cells. It may be due to the inimitable physiological function of sertoli cells and high tansfecti on efficiency of fibroblasts.