CAJ | 학술논문

以RT-PCR结合RACE方法克隆洋葱成花素同源基因AcLFT3的全长cDNA序列，利用生物信息学方法对蛋白结构进行预测分析，采用实时定量PCR法分析洋葱AcLFT3基因在不同时期不同器官的表达模式，为探讨洋葱抽薹开花的分子机制奠定基础。克隆获得的洋葱成花素同源基因AcLFT3 cDNA全长序列共838 bp，其中开放读码框为540 bp，编码由179个氨基酸组成、等电点为8.81、分子质量为20.1 ku的一个PEBP家族蛋白。AcLFT3与大葱成花素蛋白的同源性最高，为84.4%，其次为鸢尾。实时荧光定量PCR分析表明，AcLFT3基因在洋葱不同生长发育阶段器官中均有表达，以春化后抽薹前的叶片中表达量最高。
이RT-PCR결합RACE방법극륭양총성화소동원기인AcLFT3적전장cDNA서렬，이용생물신식학방법대단백결구진행예측분석，채용실시정량PCR법분석양총AcLFT3기인재불동시기불동기관적표체모식，위탐토양총추대개화적분자궤제전정기출。극륭획득적양총성화소동원기인AcLFT3 cDNA전장서렬공838 bp，기중개방독마광위540 bp，편마유179개안기산조성、등전점위8.81、분자질량위20.1 ku적일개PEBP가족단백。AcLFT3여대총성화소단백적동원성최고，위84.4%，기차위연미。실시형광정량PCR분석표명，AcLFT3기인재양총불동생장발육계단기관중균유표체，이춘화후추대전적협편중표체량최고。
The full-length cDNA of florigen homologous gene AcLFT3 was obtained by RT-PCR and Rapid Amplification of cDNA Ends. The protein structure of AcLFT3 was analyzed by bioinformatics methods. The expression pattern of AcLFT3 among organs with different developmental stages in Allium cepa were analyzed by QRT-PCR. All the results will establish a foundation for molecular mechanism of bolting and florescence in Allium cepa. In this study, the cloned cDNA sequence of AcLFT3 in Allium cepa was 838 bp with a 540 bp open reading frame (ORF). The deduced AcLFT3 protein was a member of the PEBP family, which had 179 amino acids. The molecular weight of AcLFT3 protein was 20.1 ku and its isoelectric point was 8.81. As AcLFT3 had the closest relationship to Allium fistulosum (84.4%), followed by Iris fulva. QRT-PCR result showed that AcLFT3 was expressed during all the organs with different developmental stages. More interestingly, AcLFT3 was highly expressed in leaves of Allium cepa from the stage of vernalization to bolting.