CAJ | 학술논문

以两个粳稻品种东农425和长白10为亲本，建立分别含有180、190个株系的F2：3和BC2F2群体，构建两张含有137个SSR标记的遗传图谱，对水稻粒长、粒宽、粒厚、长宽比和千粒重5个性状进行QTL定位分析。结果表明，检测到分布于水稻1、2、3、5、7、8、10、11和12号染色体上，粒长15个、粒宽8个、粒厚6个、长宽比11个、千粒重10个，共50个QTL位点。其中6个QTL qGL10、qGW3-2、qGT3-2、qL/W1-2、qTGW5和qTGW8-3的贡献率较大，为控制各性状的主效QTL。两群体定位结果相互比较，在RM1235、RM1352和RM1285标记处为粒型和粒重QTL分布的热点区域。
이량개갱도품충동농425화장백10위친본，건립분별함유180、190개주계적F2：3화BC2F2군체，구건량장함유137개SSR표기적유전도보，대수도립장、립관、립후、장관비화천립중5개성상진행QTL정위분석。결과표명，검측도분포우수도1、2、3、5、7、8、10、11화12호염색체상，립장15개、립관8개、립후6개、장관비11개、천립중10개，공50개QTL위점。기중6개QTL qGL10、qGW3-2、qGT3-2、qL/W1-2、qTGW5화qTGW8-3적공헌솔교대，위공제각성상적주효QTL。량군체정위결과상호비교，재RM1235、RM1352화RM1285표기처위립형화립중QTL분포적열점구역。
Total 180 F2:3 population lines and 190 BC2F2 population lines derived from a cross between japonica rice Dongnong425 and Changbai10,and their both linkage map including 137 SSR markers were used to map QTL control ing grain shape(grain length, grain width, grain thickness and ratio of grain length to width) and thousand-grain weight traits. The results showed that total 50 QTLs control ing the five grain traits were detected on chronosome 1, 2, 3, 5, 7, 8, 10, 11 and 12, respectively, including 15 QTLs for grain length, eight QTLs for grain width, six QTLs for grain thickness, 11 QTLs for ratio of grain length to width and 10 QTLs for thousand-grain weight. Six QTLs named qGL10, qGW3-2, qGT3-2, qL/W1-2, qTGW5 and qTGW8-3 could explain much of the observed phenotypic variance, and they were major QTLs control ing related traits. Compared the mapping QTLs results in both population, the marker named RM1235, RM1352 and RM1285 were QTLs for grain shape and weight distribution hotspots.