CAJ | 학술논문

目的：研究平肺复方对人肺腺癌A549细胞增殖及过氧化物酶体增长因子活化受体（ peroxisome proliferators-activated receptor ， PPAR）-γ信号通路的影响。方法运用细胞计数试剂盒（cell counting kit-8，CCK-8）比色法检测平肺复方对人肺腺癌A549细胞增殖的影响，并添加PPAR-γ特异性阻断剂GW9662，观察PPAR-γ通路阻断后平肺复方对A549细胞生长的影响。结果平肺复方生药5 mg/ml和10 mg/ml在作用24小时、48小时和72小时表现出对A549细胞增殖的抑制作用（ P＜0.05）；单纯加入GW9662对细胞的生长并未产生影响；加入GW9662的平肺复方仍可对细胞生长产生抑制作用（P＜0.05），但这种抑制作用明显弱于平肺复方（P＜0.05）。结论平肺复方的抗肿瘤作用机制可能部分涉及到PPAR-γ信号通路，但并不完全是通过这一条信号通路在发挥作用。
목적：연구평폐복방대인폐선암A549세포증식급과양화물매체증장인자활화수체（ peroxisome proliferators-activated receptor ， PPAR）-γ신호통로적영향。방법운용세포계수시제합（cell counting kit-8，CCK-8）비색법검측평폐복방대인폐선암A549세포증식적영향，병첨가PPAR-γ특이성조단제GW9662，관찰PPAR-γ통로조단후평폐복방대A549세포생장적영향。결과평폐복방생약5 mg/ml화10 mg/ml재작용24소시、48소시화72소시표현출대A549세포증식적억제작용（ P＜0.05）；단순가입GW9662대세포적생장병미산생영향；가입GW9662적평폐복방잉가대세포생장산생억제작용（P＜0.05），단저충억제작용명현약우평폐복방（P＜0.05）。결론평폐복방적항종류작용궤제가능부분섭급도PPAR-γ신호통로，단병불완전시통과저일조신호통로재발휘작용。
Objective To investigate the growth effect on human lung adenocarcinoma cell line A549 by Pingfei Compound , and the effect on PPAR-γSignal Pathway induced by it .Methods Prolifera-tion of A549 cells after exposure to the apozem was measured by CCK-8 assay.The PPAR-γspecificity blocker GW9662 was added .Observe if the Pingfei Compound could influence the growth of the A 549 cells or not.Results The 5 mg/mL and 10 mg/mL groups presented inhibitive effect on A 549 growth at 24h, 48h and 72h time points(P<0.05).GW9662 did not make any difference on cell growth ,while the Pingfei Compound supplemented with GW966w showed inhibitory effect on cell growth (P<0.05).Conclusion The mechanism of anti-tumor effect of Pingfei Compound may partly involved in the PPAR-γsignal path-way.But other signal pathways may meanwhile function in the anti-tumor process.