CAJ | 학술논문

在奶牛饲料中添加优良的乳酸菌微生物制剂对于生产实践有着重要的意义。由于奶牛肠道中的乳酸菌本身已适应奶牛肠道中的环境，并且与奶牛肠道中的致病菌长期拮抗竞争，因而是奶牛饲料中添加益生菌株的最佳来源。本试验利用改良MRS培养基从20头健康的荷斯坦泌乳牛粪便中进行菌群的分离鉴定，采用16SrRNA基因序列对分离的乳酸菌进行鉴定。最终获得瘤胃乳杆菌、乳酸乳球菌等18株乳酸菌。该试验中分离得到的乳酸菌为多角度开发我国乳酸菌资源和微生态制剂在畜牧生产中的应用奠定基础。
재내우사료중첨가우량적유산균미생물제제대우생산실천유착중요적의의。유우내우장도중적유산균본신이괄응내우장도중적배경，병차여내우장도중적치병균장기길항경쟁，인이시내우사료중첨가익생균주적최가래원。본시험이용개량MRS배양기종20두건강적하사탄비유우분편중진행균군적분리감정，채용16SrRNA기인서렬대분리적유산균진행감정。최종획득류위유간균、유산유구균등18주유산균。해시험중분리득도적유산균위다각도개발아국유산균자원화미생태제제재축목생산중적응용전정기출。
Cows feed with microbial adjuvant has many advantages in the production, therefore, live strains with good properties is of great signiifcance for production practice. Lactic acid bacteria in the gut itself has adapted to the gut enviroment, and the intestinal pathogenic bacteria have long-term antagonism competition relations with LAB, thus LAB become the best source of live strains. This experiment using the improved MRS culture medium to separate of bacteria from 20 lactation healthy Holstein cows dung, the lactic acid bacteria were isolated based on 16S rRNA gene sequences. Eventually there were 18 strains of lactic acid bacteria, lactic acid milk coccus isolated from the rumen lactobacillus. The lactic acid bacteria obtained from the experiment not only enriched the lactic acid bacteria species resources, but also made attribution to the development of lactic acid bacteria resources in China from multiple perspectives and gave a foundation for its application in animal production.