CAJ | 학술논문

乳酸菌是动物肠道内重要的生理性菌群，本研究以内蒙古锡林郭勒盟牧区自然放养的牛、马、山羊和绵羊为研究对象，对其肠道中的乳酸菌进行分离和16SrRNA鉴定，并对38株罗伊氏乳杆菌进行耐酸性、模拟胃肠液转运和胆盐耐受性试验。乳酸菌分离鉴定结果显示共分离到乳酸菌纯培养物96株，包括双歧杆菌属、肠球菌属、乳杆菌属和链球菌属四个菌属，其中乳杆菌属数量最多，为所有分离株的82.29%；乳杆菌属中罗伊氏乳杆菌所占比例最大，为48.10%。益生特性筛选试验结果显示菌株IMAU10240、IMAU10230和IMAU10256具有良好的耐酸、耐胃肠液和胆盐特性，可作为潜在益生菌进行进一步的益生功能研究。
유산균시동물장도내중요적생이성균군，본연구이내몽고석림곽륵맹목구자연방양적우、마、산양화면양위연구대상，대기장도중적유산균진행분리화16SrRNA감정，병대38주라이씨유간균진행내산성、모의위장액전운화담염내수성시험。유산균분리감정결과현시공분리도유산균순배양물96주，포괄쌍기간균속、장구균속、유간균속화련구균속사개균속，기중유간균속수량최다，위소유분리주적82.29%；유간균속중라이씨유간균소점비례최대，위48.10%。익생특성사선시험결과현시균주IMAU10240、IMAU10230화IMAU10256구유량호적내산、내위장액화담염특성，가작위잠재익생균진행진일보적익생공능연구。
Lactic acid bacteria is the indispensable bacteria in the gut of animal. The purpose of the study was to isolate and identify the lactic acid bacteria by 16S rRNA from the gut of cattle, horse, goat and sheep of Xilingol Inner Mongolia. Furthermore, the acid resistence, simulated gastrointestinal fluid transporting tolerance and bile salt resistence were performed on the 38 strains of Lactobacillus reuteri. The results of isolation and 16S rRNA identiifcation showed that totally 96 lactic acid bacteria were acquired, including Biifdobacterium, Enterococcus, Lactobacillus and Streptococcus, in which the Lactobacillus was the most and the proportion was 82.29%. On the isolated bacteria of Lactobacillus, Lactobacillus reuteri was the most bacteria and the proportion was 48.10%. The results of screening on the probiotics properties showed that the strains of IMAU10240,IMAU10230 and IMAU10256 were showed better tolerance to the acid of gastrointestinal lfuid and bile salt. In above, the strains of IMAU10240,IMAU10230 and IMAU10256 can serve as the potential probiotics for further research.