CAJ | 학술논문

为了探究健康牛和乳房炎患牛肠道微生物多样性及其差异性，本试验采用PCR-DGGE技术对采自内蒙古的10头健康牛及10头患病牛粪便中的细菌、乳杆菌属及纤毛虫进行分析。通过聚类分析、相似性分析及PLS-DA分析可知，健康牛和患乳房炎牛肠道微生物菌群存在明显的差异性，与健康牛相比，乳房炎患牛肠道中乳杆菌和纤毛虫多样性明显较低。PCR-DGGE技术直观全面地分析了肠道微生物菌群的多样性，为后续益生菌治疗疾病试验提供理论依据。
위료탐구건강우화유방염환우장도미생물다양성급기차이성，본시험채용PCR-DGGE기술대채자내몽고적10두건강우급10두환병우분편중적세균、유간균속급섬모충진행분석。통과취류분석、상사성분석급PLS-DA분석가지，건강우화환유방염우장도미생물균군존재명현적차이성，여건강우상비，유방염환우장도중유간균화섬모충다양성명현교저。PCR-DGGE기술직관전면지분석료장도미생물균군적다양성，위후속익생균치료질병시험제공이론의거。
In order to reveal the diversity and differences of cow with infectious mastitis and healthy cow intestinal microlfora, fecal samples of ten healthy cows and ten mastitis cows were collected in Inner Mongolia. Diversity of bacteria, Lactobacillus species and ciliate population was analyzed by denaturing gradient gel electrophoresis (DGGE). Intestinal microlfora of healthy cow and mastitis cow could be drawn with obvious differences by cluster analysis,similarity analysis and PLS-DA analysis. Compared with intestinal microflora of healthy cows, the diversity of Lactobacillus and ciliate of mastitis cows were lower. PCR-DGGE technology analyzed the diversity of intestinal microlfora and provided theoretical basis for treatment of disease by probiotic.