CAJ | 학술논문

目的：观察知母总皂苷对Aβ25～35诱导PC12细胞凋亡及凋亡相关基因Bax表达的影响。方法培养PC12细胞，实验分为6组：正常组、模型组、知母低剂量组、知母中剂量组、知母高剂量组、西药组。20μmol/L Aβ25～35作用PC12细胞48 h，诱导其凋亡。采用流式细胞仪检测各组细胞凋亡率的变化；采用RT-PCR检测各组细胞中Bax mRNA表达水平的变化；采用 Western印迹技术检测各组细胞中 Bax蛋白的表达水平的变化。结果和模型组比较，知母总皂苷能显著降低Aβ25～35诱导的凋亡（P＜0.05），能显著性降低凋亡基因Bax mRNA 和蛋白的表达水平（P＜0.05）。结论知母总皂苷能降低Aβ25～35诱导的PC12细胞凋亡率，其机制可能是降Bax mRNA及蛋白表达水平。
목적：관찰지모총조감대Aβ25～35유도PC12세포조망급조망상관기인Bax표체적영향。방법배양PC12세포，실험분위6조：정상조、모형조、지모저제량조、지모중제량조、지모고제량조、서약조。20μmol/L Aβ25～35작용PC12세포48 h，유도기조망。채용류식세포의검측각조세포조망솔적변화；채용RT-PCR검측각조세포중Bax mRNA표체수평적변화；채용 Western인적기술검측각조세포중 Bax단백적표체수평적변화。결과화모형조비교，지모총조감능현저강저Aβ25～35유도적조망（P＜0.05），능현저성강저조망기인Bax mRNA 화단백적표체수평（P＜0.05）。결론지모총조감능강저Aβ25～35유도적PC12세포조망솔，기궤제가능시강Bax mRNA급단백표체수평。
Objective To observe the effects of Anemarrhena total saponins on beta-amyloid ( Aβ)-induced PC12 cell apoptosis and the mRNA and protein expression of Bax.Methods PC12 cells was stimulated by Aβ25~35( 20μmol/L)for 48 h.Experimental groups were normal, model, Anemarrhena total saponins (5 mg/L, 10 mg/L and 20 mg/L) and the donepezil HCl groups.The apoptosis rate was ana-lyzed by fluorescence microscopy.The expression level of the Bax gene was detected by using semi-quantitative reverse transcriptase PCR ( RT-PCR).The expression levels of Bax protein was assayed by western blot.Results Anemarrhena total saponins markedly attenuated Aβ-inducing injury in PC12 cells, compared with model group (P<0.05),and it dramatically reduced the expression levels of pro-apoptotic gene Bax mRNA and protein , compared with those of model group ( P<0.05 ).Conclusions Anemarrhena total saponins treatment decrease Aβ25~35-induced the apoptosis rate of PC 12 cells, and it decreases the expression levels of Bax mRNA and protein.