CAJ | 학술논문

目的：研究食管鳞癌组织中β-catenin表达与淋巴管生成的关系。方法应用免疫组织化学SP法检测68例食管鳞癌标本及20例癌旁正常黏膜组织中β-catenin蛋白的表达状态，特异性淋巴管内皮标记物Podoplanin检测癌及正常黏膜组织的淋巴管密度（ LVD），分析β-catenin异位表达和LVD与各临床病理指标的关系，并探讨二者之间的相关性。结果①正常食管黏膜β-catenin均表达于细胞膜，而食管癌鳞组织中β-cate-nin主要呈细胞质/细胞核的异位表达，表达率为82.4％（56/68），且β-catenin 的异位表达与淋巴结转移（χ2＝7.275，P＝0.007）和外膜浸润（χ2＝6.236，P＝0.013）明显相关。②食管鳞癌组织中的LVD显著高于正常组织（t＝2.946，P＝0.002），淋巴结转移组的LVD也明显高于无淋巴结转移组（t＝7.521，P＝0.001）。③β-catenin异位表达的食管鳞癌组织与β-catenin 仅表达于细胞膜的 LVD比较差异显著（t＝2.926，P＝0.008）。结论β-catenin异位表达可能是诱导食管癌淋巴管生成的重要原因。
목적：연구식관린암조직중β-catenin표체여림파관생성적관계。방법응용면역조직화학SP법검측68례식관린암표본급20례암방정상점막조직중β-catenin단백적표체상태，특이성림파관내피표기물Podoplanin검측암급정상점막조직적림파관밀도（ LVD），분석β-catenin이위표체화LVD여각림상병리지표적관계，병탐토이자지간적상관성。결과①정상식관점막β-catenin균표체우세포막，이식관암린조직중β-cate-nin주요정세포질/세포핵적이위표체，표체솔위82.4％（56/68），차β-catenin 적이위표체여림파결전이（χ2＝7.275，P＝0.007）화외막침윤（χ2＝6.236，P＝0.013）명현상관。②식관린암조직중적LVD현저고우정상조직（t＝2.946，P＝0.002），림파결전이조적LVD야명현고우무림파결전이조（t＝7.521，P＝0.001）。③β-catenin이위표체적식관린암조직여β-catenin 부표체우세포막적 LVD비교차이현저（t＝2.926，P＝0.008）。결론β-catenin이위표체가능시유도식관암림파관생성적중요원인。
Objective To study the expression and interaction of β-catenin and Podoplanin in esophageal squamous cell carcinoma ( ESCC) , as well as the correlation with lymphangiogenesis and lymph node metastasis .Methods Immunohistochemistry staining SP was used to detect the expression level of β-catenin and Podoplanin in 68 cases of ESCC and 20 normal mucous membrane .Subsequently the re-lationship between postoperative pathological features with the expression of β-catenin and Podoplanin were analyzed .Results β-catenin was positively expressed in cell membrane of normal mucous membrane and ectopically expressed in cell muclei or cytoplasm in ESCC (56/68, 82.4%).The ectopic β-catenin expression in ESCC had significant relations with lymph mode metastasis (χ2=7.275,P=0.007)and inva-sion of adventitia(χ2=6.236,P=0.013).The LVD in ESCC was obviously higher than that in normal esophageal (t=2.946,P=0.002) and had significant relation with lymph mode metastasis (t=7.521, P=0.001).The LVD was significantly higher in ESCC with ectopicβ-catenin expression than that without ectopic β-catenin expression(t=2.926,P=0.008).Conclusions The ectopic β-catenin expression in ESCC may be the important reason of lymphangiogenesis .