CAJ | 학술논문

目的：探讨ADAM33基因的Q-1、T2位点单核苷酸多态性（ SNP）与新疆维吾尔族人群慢性阻塞性肺疾病（ COPD）发病的相关性。方法对107例新疆维吾尔族COPD患者和140例新疆维吾尔族健康体检者，采用聚合酶链反应-限制性片段长度多态性方法（ PCR-RFLP）分析AD-AM33基因Q-1位点和T2位点的基因表达多态性。结果（1）与对照组比较，COPD组 T2位点基因型分布和等位基因频率分布差异有统计学意义（均P＜0．05）。相对于GG基因型，AG＋AA基因型能够降低COPD发生的风险〔OR（95％CI）：0．536（02．93～0．983）〕。（2）单体型H4（GG）、单倍体H3（GA）和COPD存在相关性（均P＜0．05）。（3）在 COPD 组 T2位点 GG 基因型、AG＋AA 基因型的第一秒用力呼气容积占用力肺活量的百分比（ FEV1/FVC）、第一秒用力呼气容积占预计值的百分比（FEV1占预计值）差异无统计学意义（均P＞0．05）。结论在新疆维吾尔族人群中，ADAM33基因T2位点基因多态性可能与COPD发病相关。
목적：탐토ADAM33기인적Q-1、T2위점단핵감산다태성（ SNP）여신강유오이족인군만성조새성폐질병（ COPD）발병적상관성。방법대107례신강유오이족COPD환자화140례신강유오이족건강체검자，채용취합매련반응-한제성편단장도다태성방법（ PCR-RFLP）분석AD-AM33기인Q-1위점화T2위점적기인표체다태성。결과（1）여대조조비교，COPD조 T2위점기인형분포화등위기인빈솔분포차이유통계학의의（균P＜0．05）。상대우GG기인형，AG＋AA기인형능구강저COPD발생적풍험〔OR（95％CI）：0．536（02．93～0．983）〕。（2）단체형H4（GG）、단배체H3（GA）화COPD존재상관성（균P＜0．05）。（3）재 COPD 조 T2위점 GG 기인형、AG＋AA 기인형적제일초용력호기용적점용력폐활량적백분비（ FEV1/FVC）、제일초용력호기용적점예계치적백분비（FEV1점예계치）차이무통계학의의（균P＞0．05）。결론재신강유오이족인군중，ADAM33기인T2위점기인다태성가능여COPD발병상관。
Objectiev To analyze the correlation between the polymorphism of Q-1, T2 locus allele in ADAM33 gene and chronic obstructive pulmonary disease ( COPD) in Xinjiang Uygur population .Methods The genetic polymorphism of Q-1 and T2 locus allele in AD-AM33 gene in 107 Uygur patients with COPD and 140 Uygur healthy volunteers were genotyped by polymerase chain reaction -restricted frag-ment length polymorphism (PCR-RFLP).Results (1)There was significant difference in the distributions of the genotype and allele of T 2 locus between COPD and control group (all P<0.05).Compared with GG genotype,the T2 locus of AG+AA genotypes dramatically de-creased the risk of COPD〔the odds ratio OR(95%CI)=0.536(0.293~0.983)〕.When compared with G allele , A allele of T2 locus was protective for COPD〔the odds ratio OR(95%CI)=0.498(0.286~0.868)〕.(2)The haplotype H4(GG), H3(GA) were associated with COPD significantly(all P<0.05).(3) There was no significant association in the forced vital capacity ratio and the first second of forced ex -piration ratio among GG,AG+AA the genotypes of the T2 site(all P>0.05) among COPD patients.Conclusions There is correlation be-tween the polymorphism T2 locus allele in ADAM33 gene and COPD in Xinjiang Uygur population .