CAJ | 학술논문

目的：观察TPM1基因的表达与鼻咽癌(NPC)的关系。方法采用实时定量rt-PCR技术检测TPM1基因在NPC细胞株(CNE1、CNE2、58F)和鼻咽上皮细胞株(NP69)中的表达；采用组织芯片技术通过免疫组化检测TPM1在124例恶性鼻咽癌肿瘤、31例对应的癌旁正常组织和12例正常鼻咽黏膜组织的表达。结果 TPM1 mRNA在NP69中表达水平较高，而在NPC细胞株中表达水平显著下降。正常鼻咽黏膜及癌旁组织中TPM1蛋白染色阳性表达率约为65.1%(28/43)；而在鼻咽癌组织中阳性表达率约为34.7%(43/124)；TPM1蛋白表达水平与肿瘤的侵犯范围(T)呈负相关(P<0.05)，与颈淋巴结转移(N)无关(P>0.05)。结论 TPM1基因表达的抑制可能参与鼻咽癌的发生。
목적：관찰TPM1기인적표체여비인암(NPC)적관계。방법채용실시정량rt-PCR기술검측TPM1기인재NPC세포주(CNE1、CNE2、58F)화비인상피세포주(NP69)중적표체；채용조직심편기술통과면역조화검측TPM1재124례악성비인암종류、31례대응적암방정상조직화12례정상비인점막조직적표체。결과 TPM1 mRNA재NP69중표체수평교고，이재NPC세포주중표체수평현저하강。정상비인점막급암방조직중TPM1단백염색양성표체솔약위65.1%(28/43)；이재비인암조직중양성표체솔약위34.7%(43/124)；TPM1단백표체수평여종류적침범범위(T)정부상관(P<0.05)，여경림파결전이(N)무관(P>0.05)。결론 TPM1기인표체적억제가능삼여비인암적발생。
Objective To investigate the relation between TPM1 gene expression and nasopharyngeal carcinoma (NPC). Methods TPM1 expression was examined in three NPC cell lines (CNE1, CNE2 and 58F) and one nasopharyngeal epithelial cell line (NP69) by real time rt-PCR analysis and in tissue array with 124 specimens of malignant NPC, 31 NPC specimens adjacent areas and 12 normal mucosa tissues by immunohistochemistry staining. Results Compared to NP69, TPM1 mRNA expression level is significantly lower in three NPC cell lines. The positive rates of TPM1 protein were 65.1%(28/43) in NPC specimens adjacent areas and normal mucosa tissues and were 34.7%(43/124) in malignant NPC tissues. The expression of TPM1 protein in NPC was negatively related to that in degree of infiltration (T) (P<0.05) but not related to that in lymph node metastasis (N) (P>0.05). Conclusion Down-regulated of TPM1 expression may be associated with the tumorigenesis of NPC.