中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2014年
19期
3-4
,共2页
紫外分光光度法%血药浓度%美沙拉嗪
紫外分光光度法%血藥濃度%美沙拉嗪
자외분광광도법%혈약농도%미사랍진
UV-spectrophotometry%Blood concentration%Mesalazine
目的：建立测定犬血浆中美沙拉嗪浓度的紫外分光光度法(UV)。方法以5%三氯乙酸沉淀蛋白，在紫外吸收波长303 nm处测定吸光度值。结果回归方程为Y=0.0051X+0.0277(r=0.9997)，线性范围40~160μg/ml；美沙拉嗪低、中、高3种浓度方法回收率分别为(97.16±2.37)%、(98.92±1.84)%、(98.41±2.29)%；日内精密度RSD分别为2.17%、1.75%、2.21%，日间精密度RSD分别为1.88%、1.68%、1.63%。结论紫外分光光度法简单、准确、成本低，为后续开展相关研究奠定了基础。
目的：建立測定犬血漿中美沙拉嗪濃度的紫外分光光度法(UV)。方法以5%三氯乙痠沉澱蛋白，在紫外吸收波長303 nm處測定吸光度值。結果迴歸方程為Y=0.0051X+0.0277(r=0.9997)，線性範圍40~160μg/ml；美沙拉嗪低、中、高3種濃度方法迴收率分彆為(97.16±2.37)%、(98.92±1.84)%、(98.41±2.29)%；日內精密度RSD分彆為2.17%、1.75%、2.21%，日間精密度RSD分彆為1.88%、1.68%、1.63%。結論紫外分光光度法簡單、準確、成本低，為後續開展相關研究奠定瞭基礎。
목적：건립측정견혈장중미사랍진농도적자외분광광도법(UV)。방법이5%삼록을산침정단백，재자외흡수파장303 nm처측정흡광도치。결과회귀방정위Y=0.0051X+0.0277(r=0.9997)，선성범위40~160μg/ml；미사랍진저、중、고3충농도방법회수솔분별위(97.16±2.37)%、(98.92±1.84)%、(98.41±2.29)%；일내정밀도RSD분별위2.17%、1.75%、2.21%，일간정밀도RSD분별위1.88%、1.68%、1.63%。결론자외분광광도법간단、준학、성본저，위후속개전상관연구전정료기출。
Objective To establish UV-spectrophotometry (UV) method for the determination of mesalazine in dog plasma. Methods Serum protein was precipitated with 5% trichloroacetic acid, and the absorbance value at 303 nm was detected. Results The regression equation was Y=0.0051 X+0.0277 (r=0.9997), and the linear response range was 40~160μg/ml. The recovery rates of mesalazine in three different concentrations were (97.16±2.37)%, (98.92±1.84)%, (98.41±2.29)%respectively. The RSD of intra-day precision was 2.17%, 1.75%, 2.21%. The RSD of inter-day precision was 1.88%, 1.68%, 1.63%respectively. Conclusion The UV method is simple, accurate and low-cost, which can lay the foundation for the further research.
