CAJ | 학술논문

目的：研究间充质干细胞(MSC)免疫抑制功能在治疗小鼠闭塞性细支气管炎(OB)中的作用。方法分离C57BL/6小鼠骨髓MSC,建立小鼠气管移植后OB反应模型,移植当天给予MSC,30 d后处死小鼠,移植气管HE染色,观察移植气管急性排斥反应的发生情况。并用酶联免疫吸附试验(ELISA)检测移植排斥相关炎症因子的含量。结果 MSC治疗移植组与异系移植组相比,排斥反应得到缓解,与无排斥反应的同系移植对照组相似,移植气管管腔阻塞程度缓解。移植气管ELISA检测发现, MSC治疗移植组小鼠体内炎症因子IFN-g下降,而抑炎因子白细胞介素-10(IL-10)上升。结论 MSC能缓解小鼠气管移植后OB反应,并调节体内免疫状态,抑制炎症。
목적：연구간충질간세포(MSC)면역억제공능재치료소서폐새성세지기관염(OB)중적작용。방법분리C57BL/6소서골수MSC,건립소서기관이식후OB반응모형,이식당천급여MSC,30 d후처사소서,이식기관HE염색,관찰이식기관급성배척반응적발생정황。병용매련면역흡부시험(ELISA)검측이식배척상관염증인자적함량。결과 MSC치료이식조여이계이식조상비,배척반응득도완해,여무배척반응적동계이식대조조상사,이식기관관강조새정도완해。이식기관ELISA검측발현, MSC치료이식조소서체내염증인자IFN-g하강,이억염인자백세포개소-10(IL-10)상승。결론 MSC능완해소서기관이식후OB반응,병조절체내면역상태,억제염증。
Objective To analyze the effect of immunosuppression of mesenchymal stem cell (MSC) in the treatment of mice obliterans bronchiolitis (OB). Methods Marrow MSC of C57BL/6 mice was separated to set OB reaction model after trachea transplantation of mice. MSC was given on the day of transplantation. The mice were put to death after 30 days. HE staining was used on the transplanted trachea, and the situation of acute rejection of transplanted trachea was observed. Enzyme-linked immuno sorbent assay (ELISA) was applied to detect the content of inflammatory factors related to transplant rejection. Results Rejection reaction in MSC transplant group was eased, compared with different system transplant group, and degree of lumen obstruction in transplanted trachea was also eased, compared with allografts transplant control group without rejection. ELISA test for transplanted trachea showed that inflammatory factor IFN-g decreased in MSC transplant group, while anti-inflammatory factor interleukin-10 (IL-10) increased. Conclusion MSC can ease the OB reaction after trachea transplantation, adjust immune state and inhibit inflammation.