CAJ | 학술논문

目的：探讨同种异体植骨修复骨缺损的骨痂中的血小板源生长因子（PDGF）及胰岛素样生长因子（IGF-IB）基因与正常骨组织中2种生长因子基因的表达规律及机制。方法选取健康普通中国家兔30只，雌性，其中18只随机分成3 d，1、2、4、6、8周实验组，每组3只；另外10只为骨缺损异体骨松质提供组（骨提供组），2只为正常对照组；实验组均按手术要求对兔桡骨制成15 mm骨缺损模型，取骨提供组同种异体兔髂骨骨松质制成颗粒状骨移植以上骨缺损处，于术后3 d，1、2、4、6、8周处死家兔取骨缺损处标本进行PDGF和IGF-IB基因片段的实时定量聚合酶链反应（RT-PCR）；正常对照组不作处理，喂养后取相同部位正常骨组织进行实时定量聚合酶链反应。结果通过PDGF和IGF-IB的目的基因结果进行校正后，以对照组正常骨组织拷贝数为基础，实验组各时间段拷贝数与其比值：正常骨组织为1.00，实验组PDGF 3 d、1、2、4、6、8周各时间段的比值基因数分别为1.70、2.54、1.72、3.63、2.64、0.97；IGF-IB 基因比值分别是3.03、4.96、1.14、2.67、0.60、0.12。结论 PDGF和IGF-IB 2种生长因子在骨缺损修复过程中不同时间段其表达量是变化的，掌握其规律为临床上正确治疗骨缺损提供科学的理论依据。
목적：탐토동충이체식골수복골결손적골가중적혈소판원생장인자（PDGF）급이도소양생장인자（IGF-IB）기인여정상골조직중2충생장인자기인적표체규률급궤제。방법선취건강보통중국가토30지，자성，기중18지수궤분성3 d，1、2、4、6、8주실험조，매조3지；령외10지위골결손이체골송질제공조（골제공조），2지위정상대조조；실험조균안수술요구대토뇨골제성15 mm골결손모형，취골제공조동충이체토가골골송질제성과립상골이식이상골결손처，우술후3 d，1、2、4、6、8주처사가토취골결손처표본진행PDGF화IGF-IB기인편단적실시정량취합매련반응（RT-PCR）；정상대조조불작처리，위양후취상동부위정상골조직진행실시정량취합매련반응。결과통과PDGF화IGF-IB적목적기인결과진행교정후，이대조조정상골조직고패수위기출，실험조각시간단고패수여기비치：정상골조직위1.00，실험조PDGF 3 d、1、2、4、6、8주각시간단적비치기인수분별위1.70、2.54、1.72、3.63、2.64、0.97；IGF-IB 기인비치분별시3.03、4.96、1.14、2.67、0.60、0.12。결론 PDGF화IGF-IB 2충생장인자재골결손수복과정중불동시간단기표체량시변화적，장악기규률위림상상정학치료골결손제공과학적이론의거。
Objective To investigate expression regular and mechanism of platelet derived growth factor(PDGF) and in-sulin-like growth factor (IGF)-IB for repairing bone defect in allograft bone and normal bone tissue. Methods Thirty health common Chinese family female rabbits were selected,and 18 cases from which were randomized into 6 experiment groups:3 d, 1,2,4,6, 8 weeks,3 cases in each group;other 10 cases were take as support group of bone defect allogeneic cancellous bone (support group),and the other 2 cases were normal control group. The rabbit radius in the experiment groups was made to 15 mm model of bone defect according to the operation rules,the allogeneic cancellous bone from the support group was made into granu-lated cancellous bone over bone defect,and the PDGF and IGF-IB gene segments of bone defect,which were extracted from the rabbits killed on 3 d,1,2,4,6,8 weeks after operation,were conducted by real-time quantitative polymerase chain reaction (RT-PCR);the normal control group without special treatment,and the RT-PCR was adopted to the normal bone tissues in the same site after feeding. Results After proofread of PDGF and IGF-IB,the copy number and ratio of experiment groups in different time based on the copy number of normal bone tissues in control group were that the normal bone tissue was 1.00 ,the ratio of PDGF gene in the experiment group on 3 d,1,2,4,6,8 weeks was 1.70,2.54,1.72,3.63,2.64 and 0.97 respectively,while the ratio of IGF-IB gene was 3.03,4.96, 1.14,2.67,0.60 and 0.12 respectively. Conclusion The expression quantity of PDGF and IGF-IB varies at different time in the process of bone detect repair,so mastery of their regular can provide scientific theoretical basis for correct treatment of bone defect in clinic.