CAJ | 학술논문

目的：通过检测儿童哮喘急性发作时M2受体抗体的变化来探讨M2受体抗体对哮喘发作的影响。方法以合成的M2受体多肽片段为抗原，应用酶联免疫吸附测定法(ELISA)检测各组血清中M2受体抗体阳性率及抗体滴度，其中哮喘急性发作组20例，哮喘良好控制组20例，正常对照组30例。结果 M2受体抗体阳性率和抗体滴度在哮喘急性发作期组(60.0%，1：90)与正常对照组(6.7%，1：20)、哮喘良好控制组(20.0%，1：30)比较差异均有高度统计学意义(均P<0.01)。结论 M2受体抗体参与了儿童哮喘急性发作的过程并在其中起重要作用。
목적：통과검측인동효천급성발작시M2수체항체적변화래탐토M2수체항체대효천발작적영향。방법이합성적M2수체다태편단위항원，응용매련면역흡부측정법(ELISA)검측각조혈청중M2수체항체양성솔급항체적도，기중효천급성발작조20례，효천량호공제조20례，정상대조조30례。결과 M2수체항체양성솔화항체적도재효천급성발작기조(60.0%，1：90)여정상대조조(6.7%，1：20)、효천량호공제조(20.0%，1：30)비교차이균유고도통계학의의(균P<0.01)。결론 M2수체항체삼여료인동효천급성발작적과정병재기중기중요작용。
Objective To investigate the effects of autoantibodies against M2-muscarinic receptor in acute attack of bronchial asthma in children. Methods The positive rate of autoantibodies against M2-muscarinic receptor and the an-tibody titer in the serum were detected by ELISA using synthesized peptide fragment of M2-muscarinic receptor in 3 groups: asthma acute attack (n = 20), asthma well control (n = 20), and normal control (n = 30). Results The positive rate of autoantibodies against M2-muscarinic receptor and the antibody titer in the serum from acute asthma group (60%, 1:90) were much higher than those from normal control group (6.7%, 1:20, P< 0.01) and asthma well control group (20%, 1:30, P< 0.01). Conclusion There is a close relationship between the autoantibodies against M2-mus-carinic receptor and the pathogenetic condition of asthma in children, it may play a very important role in the develop-ment of asthma.