重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
27期
3584-3587
,共4页
前列腺肿瘤%细胞增殖%Cyclopamine
前列腺腫瘤%細胞增殖%Cyclopamine
전렬선종류%세포증식%Cyclopamine
prostate neoplasms%cell proliferation%Cyclopamine
目的：探讨Cyclopamine对前列腺癌LNCaP细胞增殖、凋亡的作用及对PSAmRNA基因表达的影响。方法不同水平Cyclopamine（1、5、10、15μmol/L）干预LNCaP细胞不同时间（24、48、72 h），MTT法检测其对细胞增殖的抑制，Hoechst33258染色观察细胞形态变化，流式细胞术检测细胞凋亡率，实时荧光定量逆转录聚合酶链反应（FQ-RT-PCR）观察PSAmRNA基因表达的影响。结果5、10、15μmol/L组Cyclopamine对LNCaP细胞增殖有显著抑制作用，与对照组比较差异有统计学意义（P＜0．01），10μmol/L组于48 h达到IC50；10、15μmol/L组的24、48、72 h凋亡率分别为37．21％、57．38％、57．98％和21．16％、71．31％、72．90％，与对照组比较差异有统计学意义（P＜0．01）。细胞凋亡形态的改变随着 Cyclopamine水平增大和作用时间的延长也显著增强。PSAmRNA基因的表达水平随着水平的上升呈现明显的递减趋势，较对照组显著降低（P＜0．01）；10、15μmol/L水平的Cyclopamine在不同时间段PSAmRNA基因的表达均极低。结论 Cyclopamine能够明显抑制 LNCaP细胞的增殖，诱导细胞凋亡，并显著下调LNCaP细胞PSAmRNA基因的表达，一定水平Cyclopamine可能对晚期前列腺癌的治疗有效。
目的：探討Cyclopamine對前列腺癌LNCaP細胞增殖、凋亡的作用及對PSAmRNA基因錶達的影響。方法不同水平Cyclopamine（1、5、10、15μmol/L）榦預LNCaP細胞不同時間（24、48、72 h），MTT法檢測其對細胞增殖的抑製，Hoechst33258染色觀察細胞形態變化，流式細胞術檢測細胞凋亡率，實時熒光定量逆轉錄聚閤酶鏈反應（FQ-RT-PCR）觀察PSAmRNA基因錶達的影響。結果5、10、15μmol/L組Cyclopamine對LNCaP細胞增殖有顯著抑製作用，與對照組比較差異有統計學意義（P＜0．01），10μmol/L組于48 h達到IC50；10、15μmol/L組的24、48、72 h凋亡率分彆為37．21％、57．38％、57．98％和21．16％、71．31％、72．90％，與對照組比較差異有統計學意義（P＜0．01）。細胞凋亡形態的改變隨著 Cyclopamine水平增大和作用時間的延長也顯著增彊。PSAmRNA基因的錶達水平隨著水平的上升呈現明顯的遞減趨勢，較對照組顯著降低（P＜0．01）；10、15μmol/L水平的Cyclopamine在不同時間段PSAmRNA基因的錶達均極低。結論 Cyclopamine能夠明顯抑製 LNCaP細胞的增殖，誘導細胞凋亡，併顯著下調LNCaP細胞PSAmRNA基因的錶達，一定水平Cyclopamine可能對晚期前列腺癌的治療有效。
목적：탐토Cyclopamine대전렬선암LNCaP세포증식、조망적작용급대PSAmRNA기인표체적영향。방법불동수평Cyclopamine（1、5、10、15μmol/L）간예LNCaP세포불동시간（24、48、72 h），MTT법검측기대세포증식적억제，Hoechst33258염색관찰세포형태변화，류식세포술검측세포조망솔，실시형광정량역전록취합매련반응（FQ-RT-PCR）관찰PSAmRNA기인표체적영향。결과5、10、15μmol/L조Cyclopamine대LNCaP세포증식유현저억제작용，여대조조비교차이유통계학의의（P＜0．01），10μmol/L조우48 h체도IC50；10、15μmol/L조적24、48、72 h조망솔분별위37．21％、57．38％、57．98％화21．16％、71．31％、72．90％，여대조조비교차이유통계학의의（P＜0．01）。세포조망형태적개변수착 Cyclopamine수평증대화작용시간적연장야현저증강。PSAmRNA기인적표체수평수착수평적상승정현명현적체감추세，교대조조현저강저（P＜0．01）；10、15μmol/L수평적Cyclopamine재불동시간단PSAmRNA기인적표체균겁저。결론 Cyclopamine능구명현억제 LNCaP세포적증식，유도세포조망，병현저하조LNCaP세포PSAmRNA기인적표체，일정수평Cyclopamine가능대만기전렬선암적치료유효。
Objective To investigate the effects of cyclopamine on the proliferation ,apoptosis and the expression of PSAmRNA in prostate cancer LNCaP cells .Methods LNCaP cells were interfered with different concentrations of cyclopamine (1 ,5 ,10 ,15μmol/L) at the different timepoints(24 ,48 ,72 h) .The poliferation inhibition was measured by the MTT assay ;the apoptotic mor-phological changes were observed by the Hoechst33258 staining method ;the apoptosis rate was examined by the flow cytometry ;the effects of PSAmRNA gene expression was detected by the FQ-RT-PCR .Results 5 ,10 ,15μmol/L cyclopamine groups had obvious inhibition effect on the LNCaP cell proliferation ,which had statistically significant difference compared with the control group (P<0 .01) .10 μmol/L group reached IC50 at 48 h ;the apoptosis rates at 24 ,48 ,72 h in the 10 ,15 μmol/L groups were 37 .21% , 57 .38% ,57 .98% and 21 .16% ,71 .31% ,72 .90% respectively ,the difference had statistically significant difference compared with the control group(P<0 .01) .The cellular apoptotic morphological changes were significantly enhanced with the increase of cyclo-pamine concentration and the extension of action time .The expression level PSAmRNA gene exhibited the obvious decreasing trend with the increase of cyclopamine concentration and was significantly decreased compared with the control group (P< 0 .01) .The PSAmRNA gene expression was extremely low in 10 ,15 μmol/L cyclopamine at different time period .Conclusion Cyclopamine significantly inhibits the proliferation of LNCaP cells ,induces apoptosis and obviously down-regulates the PSAmRNA gene expres-sion in LNCaP cells .Certain concentration of cyclopamine may be effective for treating advanced prostate cancer .
