中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2014年
28期
110-112
,共3页
胸腹水%液基薄层细胞制片%免疫细胞化学染色
胸腹水%液基薄層細胞製片%免疫細胞化學染色
흉복수%액기박층세포제편%면역세포화학염색
Hydrothorax and ascite%Liquid based thin layer cell production%Immunocytochemistry staining
目的:探讨液基薄层细胞检测和传统涂片在胸腹水细胞病理诊断中的意义以及免疫细胞化学染色在恶性胸腹水诊断中的价值。方法:收集胸腹水标本236例,进行两种方法的制片,HE 染色,并进行 CEA、CK5/6、CK7、Ki-67免疫细胞化学染色。结果:液基细胞检测技术检测胸腹水恶性肿瘤检出率高于传统涂片技术,两组间差异有统计学意义(P<0.05);恶性胸腹水CEA、CK5/6、CK7、Ki-67表达阳性率高于良性胸腹水,两组间差异有统计学意义(P均<0.01)。结论:液基细胞检测技术检测胸腹水恶性肿瘤检出率高于传统涂片技术,辅以免疫细胞化学检测,对肿瘤的诊断、鉴别诊断及细胞来源有重要的价值。
目的:探討液基薄層細胞檢測和傳統塗片在胸腹水細胞病理診斷中的意義以及免疫細胞化學染色在噁性胸腹水診斷中的價值。方法:收集胸腹水標本236例,進行兩種方法的製片,HE 染色,併進行 CEA、CK5/6、CK7、Ki-67免疫細胞化學染色。結果:液基細胞檢測技術檢測胸腹水噁性腫瘤檢齣率高于傳統塗片技術,兩組間差異有統計學意義(P<0.05);噁性胸腹水CEA、CK5/6、CK7、Ki-67錶達暘性率高于良性胸腹水,兩組間差異有統計學意義(P均<0.01)。結論:液基細胞檢測技術檢測胸腹水噁性腫瘤檢齣率高于傳統塗片技術,輔以免疫細胞化學檢測,對腫瘤的診斷、鑒彆診斷及細胞來源有重要的價值。
목적:탐토액기박층세포검측화전통도편재흉복수세포병리진단중적의의이급면역세포화학염색재악성흉복수진단중적개치。방법:수집흉복수표본236례,진행량충방법적제편,HE 염색,병진행 CEA、CK5/6、CK7、Ki-67면역세포화학염색。결과:액기세포검측기술검측흉복수악성종류검출솔고우전통도편기술,량조간차이유통계학의의(P<0.05);악성흉복수CEA、CK5/6、CK7、Ki-67표체양성솔고우량성흉복수,량조간차이유통계학의의(P균<0.01)。결론:액기세포검측기술검측흉복수악성종류검출솔고우전통도편기술,보이면역세포화학검측,대종류적진단、감별진단급세포래원유중요적개치。
Objective:To explore the significances of thinprep cytology test and conventional smea in the pathological diagnosis of hydrothorax and ascite cells,and the value of immunocytochemical staining in the diagnosis of malignant pleural effusion and ascites.Methods:236 cases of hydrothorax and ascite samples were selected.They were given two methods of production,HE staining,and CEA,CK5/6,CK7,Ki-67 immunocytochemical staining.Results:The detection rate of liquid based cell detection technology in the detection of hydrothorax and ascite malignant tumor was higher than that of conventional smear technique,and there was significant difference between two groups(P<0.05).The expression positive rate of CEA、CK5/6、CK7、Ki-67 in the malignant pleural effusion and ascites were higher than that of the benign hydrothorax and ascites,and there was significant difference between two groups(P<0.01).Conclusion:The detection rate of liquid based cell detection technology in the detection of hydrothorax and ascite malignant tumor was higher than that of conventional smear technique,supplemented by immunohistochemistry.It has important value on the tumor diagnosis, differential diagnosis and cell source.