CAJ | 학술논문

目的：检测胃癌患者血管内皮因子C（VEGF-C）蛋白及mRNA在胃癌组织中的表达情况，探讨其与胃癌淋巴结微转移的关系。方法应用免疫组织化学法及RT-PCR法检测80例胃癌组织、癌旁组织及正常组织中的VEGF-C蛋白及mRNA表达情况；免疫组化法检测淋巴结微转移情况，比较有无淋巴结微转移的VEGF-C蛋白和mRNA的表达差异，明确存在的关联性。结果VEGF-C蛋白及mRNA两者在胃癌组织中明显高于癌旁组织和正常组织（P＜0.05）。结论胃癌组织中的VEGF-C蛋白及mRNA与胃癌淋巴结微转移相关，基因检测优于蛋白，可作为评价胃癌患者是否存在淋巴结微转移的优选指标。
목적：검측위암환자혈관내피인자C（VEGF-C）단백급mRNA재위암조직중적표체정황，탐토기여위암림파결미전이적관계。방법응용면역조직화학법급RT-PCR법검측80례위암조직、암방조직급정상조직중적VEGF-C단백급mRNA표체정황；면역조화법검측림파결미전이정황，비교유무림파결미전이적VEGF-C단백화mRNA적표체차이，명학존재적관련성。결과VEGF-C단백급mRNA량자재위암조직중명현고우암방조직화정상조직（P＜0.05）。결론위암조직중적VEGF-C단백급mRNA여위암림파결미전이상관，기인검측우우단백，가작위평개위암환자시부존재림파결미전이적우선지표。
Objective To explore the expression of vascular endothelial growth factor-C (VEGF-C) protein and mRNA in gastric cancer and its relationship with lymph node micrometastases. Methods Immunohistochemistry and RT PCR were used to detect the expression of VEGF-C protein and mRNA in gastric carcinoma tissue, adjacent tissue and normal tissue from 80 patients who suffered from gastric carcinoma. The expression level of VEGF-C protein and mRNA were compared between tissues with positive or negative lymph node micrometastases. Results The positive expression rates of VEGF-C protein and mRNA in gastric cancer tissue were significantly higher than those in adjacent tissue and normal tissue(P< 0.05), which had no relationship with the patients' age, sex, tumor size, location and distant metastasis. The expression of VEGF-C was positively correlated with lymph node metastasis(P< 0.01), and that of VEGF-C mRNA with micrometastasis was closer than that of protein. Conclusions VEGF-C protein and mRNA in gastric cancer tissue is correlated with lymph node micrometastases, and detection of VEGF-C mRNA is prior to detection of protein. VEGF-C mRNA can be serving as a more accurate indicator of lymph node micrometastases in the gastric cancer patients.