中华普通外科学文献（电子版）
中華普通外科學文獻（電子版）
중화보통외과학문헌（전자판）
CHINESE JOURNAL OF GENERAL SURGERY(ELECTRONIC VERSION)
2014年
5期
365-369
,共5页
李新丰%周文瑞%王高雄%李承中%黄天从
李新豐%週文瑞%王高雄%李承中%黃天從
리신봉%주문서%왕고웅%리승중%황천종
高尔基体糖蛋白73%甲胎蛋白%血管内皮生长因子%原发性肝癌
高爾基體糖蛋白73%甲胎蛋白%血管內皮生長因子%原髮性肝癌
고이기체당단백73%갑태단백%혈관내피생장인자%원발성간암
Golgi protein 73%Alpha-fetoproteins%Vascular endothelial growth factor%Primary hepatic carcinoma
目的：研究高尔基体糖蛋白73（GP73）、甲胎蛋白（AFP）、血管内皮生长因子（VEGF）在原发性肝癌（PHC）患者血清中的表达意义。方法43例PHC及9例肝良性肿瘤患者接受双抗体夹心ELISA法检测血清GP73、VEGF浓度及电化学发光法检测血清AFP浓度。结果 GP73在不同年龄、性别、肿瘤大小间的表达差异无统计学意义。伴乙肝病毒携带的患者其GP73表达水平高于不伴有乙肝病毒携带的患者（P＜0.05），伴有肝硬化的患者其GP73表达水平高于不伴有肝硬化的患者（P＜0.05）。PHC组高于肝良性肿瘤组[（241.413±77.079）μg/L vs（101.866±74.192）μg/L，P＜0.01]。手术前GP73、AFP和VEGF表达水平均高于手术后[（674.176±1090.083）μg/Lvs（178.560±289.330）μg/L，（256.666±164.760）μg/L vs（149.072±158.643）μg/L，P＜0.01]。AFP、GP73、VEGF在PHC鉴别诊断的ROC曲线下面积分别为0.894、0.791、0.612，GP73面积为最大。GP73诊断PHC组敏感性及特异性为88.4%、77.7%，高于AFP。将AFP及GP73并联检测后发现，敏感度为96.8%，特异度为63.7%，与单项检测相比，GP73联合AFP可明显提高PHC诊断的准确性。结论 GP73是一种灵敏度、特异度更高的PHC诊断的血清标志物，而GP73、AFP联合检测可提高PHC诊断准确性。
目的：研究高爾基體糖蛋白73（GP73）、甲胎蛋白（AFP）、血管內皮生長因子（VEGF）在原髮性肝癌（PHC）患者血清中的錶達意義。方法43例PHC及9例肝良性腫瘤患者接受雙抗體夾心ELISA法檢測血清GP73、VEGF濃度及電化學髮光法檢測血清AFP濃度。結果 GP73在不同年齡、性彆、腫瘤大小間的錶達差異無統計學意義。伴乙肝病毒攜帶的患者其GP73錶達水平高于不伴有乙肝病毒攜帶的患者（P＜0.05），伴有肝硬化的患者其GP73錶達水平高于不伴有肝硬化的患者（P＜0.05）。PHC組高于肝良性腫瘤組[（241.413±77.079）μg/L vs（101.866±74.192）μg/L，P＜0.01]。手術前GP73、AFP和VEGF錶達水平均高于手術後[（674.176±1090.083）μg/Lvs（178.560±289.330）μg/L，（256.666±164.760）μg/L vs（149.072±158.643）μg/L，P＜0.01]。AFP、GP73、VEGF在PHC鑒彆診斷的ROC麯線下麵積分彆為0.894、0.791、0.612，GP73麵積為最大。GP73診斷PHC組敏感性及特異性為88.4%、77.7%，高于AFP。將AFP及GP73併聯檢測後髮現，敏感度為96.8%，特異度為63.7%，與單項檢測相比，GP73聯閤AFP可明顯提高PHC診斷的準確性。結論 GP73是一種靈敏度、特異度更高的PHC診斷的血清標誌物，而GP73、AFP聯閤檢測可提高PHC診斷準確性。
목적：연구고이기체당단백73（GP73）、갑태단백（AFP）、혈관내피생장인자（VEGF）재원발성간암（PHC）환자혈청중적표체의의。방법43례PHC급9례간량성종류환자접수쌍항체협심ELISA법검측혈청GP73、VEGF농도급전화학발광법검측혈청AFP농도。결과 GP73재불동년령、성별、종류대소간적표체차이무통계학의의。반을간병독휴대적환자기GP73표체수평고우불반유을간병독휴대적환자（P＜0.05），반유간경화적환자기GP73표체수평고우불반유간경화적환자（P＜0.05）。PHC조고우간량성종류조[（241.413±77.079）μg/L vs（101.866±74.192）μg/L，P＜0.01]。수술전GP73、AFP화VEGF표체수평균고우수술후[（674.176±1090.083）μg/Lvs（178.560±289.330）μg/L，（256.666±164.760）μg/L vs（149.072±158.643）μg/L，P＜0.01]。AFP、GP73、VEGF재PHC감별진단적ROC곡선하면적분별위0.894、0.791、0.612，GP73면적위최대。GP73진단PHC조민감성급특이성위88.4%、77.7%，고우AFP。장AFP급GP73병련검측후발현，민감도위96.8%，특이도위63.7%，여단항검측상비，GP73연합AFP가명현제고PHC진단적준학성。결론 GP73시일충령민도、특이도경고적PHC진단적혈청표지물，이GP73、AFP연합검측가제고PHC진단준학성。
Objective To investigate the expression and clinical significance of serum Golgi protein 73 (GP73) , Alpha-fetoproteins (AFP) and vascular endothelial growth factor (VEGF) in primary hepatic cancer (PHC) patients. Methods Serum AFP, GP73 and VEGF levels of forty-three PHC patients and 9 patients with benign hepatic tumor were detected by electrochemiluminescence and double antibody sandwich ELISA. Results The serum levels of GP73 in different ages, gender, tumor sizes was not significantly different. The serum level of GP73 in hepatitis B virus carriers was significantly higher than no hepatitis B virus carriers, and that in hepatic cirrhosis group was significantly higher than no cirrhosis group (P<0.05) . The serum level of GP73 in PHC group was significantly higher than benign liver tumor group[ (241.413±77.079) vs (101.866±74.192) μg/L, P<0.01]. The serum levels of GP73 , AFP and VEGF before the operation were significantly higher than those after the operation [(247.231±77.009)μg/L vs (128.334±92.235)μg/L, (674.176±1 090.083)μg/L vs(178.560±289.330)μg/L,(256.666±164.76)μg/L vs ( 1 4 9 . 0 7 2 ± 1 5 8 . 6 4 3 ) μg/L , all P<0.01]. The area of AFP, GP73, VEGF under the ROC curve in the differential diagnosis of PHC were 0.894, 0.791, 0.612. The sensitivity and specificity value of GP73 were 88.4%and 77.7%, higher than those of AFP. The sensitivity and specificity value of AFP combined with GP73 reached 96.8%and 63.7%, higher than either single detection. Conclusion GP73 is a sensitivity and specificity PHC diagnostic serum markers. AFP combined with GP73 detection has higher accuracy for PHC diagnosis.
